Platelets lysate-based membranes for periodontal ligament regeneration

Bibliographic Details
Main Author: Babo, P. M.
Publication Date: 2013
Other Authors: Santo, Vítor E., Duarte, Ana Rita C., Gomes, Manuela E., Reis, R. L.
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/1822/27075
Summary: Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013)
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spelling Platelets lysate-based membranes for periodontal ligament regenerationGrowth factors deliveryMembranesPeriodontal ligamentPlatelet lysatePublicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013)The periodontal ligament (PDL) is a group of specialized connective tissue fibers that attach a tooth to the alveolar bone where it is deployed. These fibers help the tooth withstand the substantial compressive forces which occur during chewing and remain embedded in the bone. Periodontitis is a prevalent infection disease that causes the destruction of the tooth supportive tissues including the PDL. Given its low ability of regeneration in adult patients, concerted efforts have been made to accelerate periodontal tissue regeneration. Nevertheless, a strategy for predictable reconstruction of normal structure and functionality of periodontal damaged tissue is yet to be achieved. In this work, we present a novel membrane based on platelets lysate (PL) aiming for PDL regeneration. PL is a source of multiple growth factors (GFs) such as PDGF-BB, VEGF, and TGF-β1, which are prompt to induce wound healing and the recruitment of cells for tissue regeneration. In this work, we propose the development of PL-based membranes prepared by crosslinking PL proteins with genipin for periodontal tissue regeneration. Increasing concentrations of genipin (0.10, 0.18 and 0.25 % w/v) were used to crosslink PL proteins to produce PL-based membranes. The resulting membranes showed increasing crosslinking density proportional to the crosslinker concentration. In addition, the morphological and mechanical features have shown to be dependent on the crosslinking degree of the PL membranes. The release of specific GFs was quantified by ELISA. Results show that the produced membranes are able to release the GFs contained in PL in a controlled manner and proportional to the crosslinking density, with a higher cumulative release for the samples with lower crosslinking density. In vitro assays were performed both using human adipose derived stem cells (hASCs) and periodontal ligament fibroblasts (hPDLFs). While no significant proliferation was detected when using hASCs, the hPDLFs showed good adhesion and proliferation on the membranes, suggesting its compatibility with PDL regeneration approaches. The PL-based membranes developed in this work, present high stiffness and elasticity and, consequently, a great potential in the regeneration of elastic and mechanically active tissues. Moreover these membranes have demonstrated to act as a valuable substrate for hPDLFs attachment and growth in 2D conditions and provide an environment rich in GFs with a major role in wound healing. These results suggest that it is possible to produce stable PL-based membranes crosslinked with genipin and that these membranes have great potential for future applications in the regeneration of PDL.John Wiley and SonsUniversidade do MinhoBabo, P. M.Santo, Vítor E.Duarte, Ana Rita C.Gomes, Manuela E.Reis, R. L.2013-102013-10-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/27075enghttp://onlinelibrary.wiley.com/doi/10.1002/term.2013.7.issue-s1/issuetocinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T04:26:13Zoai:repositorium.sdum.uminho.pt:1822/27075Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T14:48:18.305863Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Platelets lysate-based membranes for periodontal ligament regeneration
title Platelets lysate-based membranes for periodontal ligament regeneration
spellingShingle Platelets lysate-based membranes for periodontal ligament regeneration
Babo, P. M.
Growth factors delivery
Membranes
Periodontal ligament
Platelet lysate
title_short Platelets lysate-based membranes for periodontal ligament regeneration
title_full Platelets lysate-based membranes for periodontal ligament regeneration
title_fullStr Platelets lysate-based membranes for periodontal ligament regeneration
title_full_unstemmed Platelets lysate-based membranes for periodontal ligament regeneration
title_sort Platelets lysate-based membranes for periodontal ligament regeneration
author Babo, P. M.
author_facet Babo, P. M.
Santo, Vítor E.
Duarte, Ana Rita C.
Gomes, Manuela E.
Reis, R. L.
author_role author
author2 Santo, Vítor E.
Duarte, Ana Rita C.
Gomes, Manuela E.
Reis, R. L.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Babo, P. M.
Santo, Vítor E.
Duarte, Ana Rita C.
Gomes, Manuela E.
Reis, R. L.
dc.subject.por.fl_str_mv Growth factors delivery
Membranes
Periodontal ligament
Platelet lysate
topic Growth factors delivery
Membranes
Periodontal ligament
Platelet lysate
description Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013)
publishDate 2013
dc.date.none.fl_str_mv 2013-10
2013-10-01T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/27075
url http://hdl.handle.net/1822/27075
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://onlinelibrary.wiley.com/doi/10.1002/term.2013.7.issue-s1/issuetoc
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley and Sons
publisher.none.fl_str_mv John Wiley and Sons
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833594897947426816

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