Modulation of rye rDNA chromatin conformation induced by high temperature stress

Bibliographic Details
Main Author: Brazão, João Miguel Henriques da Silva
Publication Date: 2010
Format: Report
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10400.5/2973
Summary: Report - Bolsa de integração na Investigação
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spelling Modulation of rye rDNA chromatin conformation induced by high temperature stressryerDNAchromatinstressReport - Bolsa de integração na InvestigaçãoEpigenetic modulation has been correlated with expression patterns alterations involved in plant response to stress. However, the influence of abiotic stress, namely high temperature, in chromatin organization of plant nuclei is poorly known. The aim of this work was the analysis of rDNA chromatin organization in interphase nuclei of rye root-tip and coleoptiles from high temperature treated (HTS) and untreated plantlets, through Fluorescent In Situ Hybridization (FISH) using rDNA probes. Rye seeds were germinated in controlled conditions and HTS treated plantlets were subject to a period of 4 hours at 40oC. FISH was performed successfully using a new rDNA probe produced by PCR that can replace with advantage the pTa71 probe and the results obtained with both probes revealed significant differences in rDNA chromatin organization patterns between control and HTS in interphase nuclei from both tissues analyzed. The detected rDNA chromatin decondensation provoked by HTS may be involved in a greater potential for transcription necessary to the amendment induced by HTS and emphasize the role of chromatin epigenetic remodelling in plant adjustment pathways under stress conditions.ISARepositório da Universidade de LisboaBrazão, João Miguel Henriques da Silva2011-03-18T14:40:54Z2010-01-042010-01-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/reportapplication/pdfhttp://hdl.handle.net/10400.5/2973engBrazão, J.M.H.S. - Modulation of rye rDNA chromatin conformation induced by high temperature stress. Lisboa: ISA/CBAA, 2010info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T16:00:46Zoai:repositorio.ulisboa.pt:10400.5/2973Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T04:00:00.252760Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Modulation of rye rDNA chromatin conformation induced by high temperature stress
title Modulation of rye rDNA chromatin conformation induced by high temperature stress
spellingShingle Modulation of rye rDNA chromatin conformation induced by high temperature stress
Brazão, João Miguel Henriques da Silva
rye
rDNA
chromatin
stress
title_short Modulation of rye rDNA chromatin conformation induced by high temperature stress
title_full Modulation of rye rDNA chromatin conformation induced by high temperature stress
title_fullStr Modulation of rye rDNA chromatin conformation induced by high temperature stress
title_full_unstemmed Modulation of rye rDNA chromatin conformation induced by high temperature stress
title_sort Modulation of rye rDNA chromatin conformation induced by high temperature stress
author Brazão, João Miguel Henriques da Silva
author_facet Brazão, João Miguel Henriques da Silva
author_role author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Brazão, João Miguel Henriques da Silva
dc.subject.por.fl_str_mv rye
rDNA
chromatin
stress
topic rye
rDNA
chromatin
stress
description Report - Bolsa de integração na Investigação
publishDate 2010
dc.date.none.fl_str_mv 2010-01-04
2010-01-04T00:00:00Z
2011-03-18T14:40:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/2973
url http://hdl.handle.net/10400.5/2973
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazão, J.M.H.S. - Modulation of rye rDNA chromatin conformation induced by high temperature stress. Lisboa: ISA/CBAA, 2010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ISA
publisher.none.fl_str_mv ISA
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833601906015993856

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