Phenotypic analysis of urothelial exfoliated cells in bladder cancer via microfluidic immunoassays: Sialyl-Tn as a novel biomarker in liquid biopsies

• Main Author: Carvalho, Sandra
• Publication Date: 2020
• Other Authors: Abreu, Catarina M., Ferreira, Dylan, Lima, Luís, Santos, Lúcio L., Ribeiro, Ricardo, Grenha, Vânia, Martínez-Fernández, Mónica, Duenas, Marta, Suárez-Cabrera, Cristian, Paramio, Jesus M., Diéguez, Lorena, Freitas, Paulo P., Oliveira, Marta I.
• Format: Article
• Language: eng
• Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
• Download full: http://hdl.handle.net/10400.22/16517
• topic: Bladder cancer; Microfluidics; Liquid biopsy; Urine; Sialyl-TnMM

Bladder cancer is the most common malignancy of the urinary tract, having one of the highest recurrence rates and progression from non-muscle to muscle invasive bladder cancer that commonly leads to metastasis. Cystoscopy and urine cytology are the standard procedures for its detection but have limited clinical sensitivity and specificity. Herein, a microfluidic device, the UriChip, was developed for the enrichment of urothelial exfoliated cells from fresh and frozen urine, based on deformability and size, and the cancer-associated glycan Sialyl-Tn explored as a putative bladder cancer urinary biomarker.