A further investigation of the cytochrome b5–cytochrome c complex

Bibliographic Details
Main Author: Banci, Lucia
Publication Date: 2003
Other Authors: Bertini, Ivano, Felli, Isabella C., Krippahl, Ludwig, Kubicek, Karel, Moura, José J. G., Rosato, Antonio
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/1660
Summary: J Biol Inorg Chem (2003) 8: 777–786
id RCAP_3c97f1b5e130d618a932152d1f29815f
oai_identifier_str oai:run.unl.pt:10362/1660
network_acronym_str RCAP
network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository_id_str https://opendoar.ac.uk/repository/7160
spelling A further investigation of the cytochrome b5–cytochrome c complexCytochrome b5Cytochrome cElectron transferProtein–protein interactionProtein recognitionJ Biol Inorg Chem (2003) 8: 777–786The interaction of reduced rabbit cytochrome b5 with reduced yeast iso-1 cytochrome c has been studied through the analysis of 1H–15N HSQC spectra, of 15N longitudinal (R1) and transverse (R2) relaxation rates, and of the solvent exchange rates of protein backbone amides. For the first time, the adduct has been investigated also from the cytochrome c side. The analysis of the NMR data was integrated with docking calculations. The result is that cytochrome b5 has two negative patches capable of interacting with a single positive surface area of cytochrome c. At low protein concentrations and in equimolar mixture, two different 1:1 adducts are formed. At high concentration and/or with excess cytochrome c, a 2:1 adduct is formed. All the species are in fast exchange on the scale of differences in chemical shift. By comparison with literature data, it appears that the structure of one 1:1 adduct changes with the origin or primary sequence of cytochrome b5.SBICRUNBanci, LuciaBertini, IvanoFelli, Isabella C.Krippahl, LudwigKubicek, KarelMoura, José J. G.Rosato, Antonio2008-09-24T14:00:45Z20032003-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/1660eng1432-1327info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:06:18Zoai:run.unl.pt:10362/1660Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:37:10.485934Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv A further investigation of the cytochrome b5–cytochrome c complex
title A further investigation of the cytochrome b5–cytochrome c complex
spellingShingle A further investigation of the cytochrome b5–cytochrome c complex
Banci, Lucia
Cytochrome b5
Cytochrome c
Electron transfer
Protein–protein interaction
Protein recognition
title_short A further investigation of the cytochrome b5–cytochrome c complex
title_full A further investigation of the cytochrome b5–cytochrome c complex
title_fullStr A further investigation of the cytochrome b5–cytochrome c complex
title_full_unstemmed A further investigation of the cytochrome b5–cytochrome c complex
title_sort A further investigation of the cytochrome b5–cytochrome c complex
author Banci, Lucia
author_facet Banci, Lucia
Bertini, Ivano
Felli, Isabella C.
Krippahl, Ludwig
Kubicek, Karel
Moura, José J. G.
Rosato, Antonio
author_role author
author2 Bertini, Ivano
Felli, Isabella C.
Krippahl, Ludwig
Kubicek, Karel
Moura, José J. G.
Rosato, Antonio
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Banci, Lucia
Bertini, Ivano
Felli, Isabella C.
Krippahl, Ludwig
Kubicek, Karel
Moura, José J. G.
Rosato, Antonio
dc.subject.por.fl_str_mv Cytochrome b5
Cytochrome c
Electron transfer
Protein–protein interaction
Protein recognition
topic Cytochrome b5
Cytochrome c
Electron transfer
Protein–protein interaction
Protein recognition
description J Biol Inorg Chem (2003) 8: 777–786
publishDate 2003
dc.date.none.fl_str_mv 2003
2003-01-01T00:00:00Z
2008-09-24T14:00:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/1660
url http://hdl.handle.net/10362/1660
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1432-1327
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv SBIC
publisher.none.fl_str_mv SBIC
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833596061682237440

Similar Items