A wide-spectrum antimicrobial coating to fight multi-species biofilms in ventilator-associated pneumonia infections

Bibliographic Details
Main Author: Alves, Diana Filipa Barros
Publication Date: 2021
Other Authors: Pereira, Maria Olívia, Lopes, Susana Patrícia
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/1822/75660
Summary: This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. The authors also acknowledge the support, through the Programa Operacional Competitividade e Internacionalização (COMPETE2020) and by national funds, through the Portuguese Foundation for Science and Technology (FCT), of the POLY-PrevEnTT project (PTDC/BTM-SAL/29841/2017-POCI-01-0145-FEDER-029841).
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spelling A wide-spectrum antimicrobial coating to fight multi-species biofilms in ventilator-associated pneumonia infectionsdual-drug release coatingventilator-associated pneumoniamulti-species biofilmsThis study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. The authors also acknowledge the support, through the Programa Operacional Competitividade e Internacionalização (COMPETE2020) and by national funds, through the Portuguese Foundation for Science and Technology (FCT), of the POLY-PrevEnTT project (PTDC/BTM-SAL/29841/2017-POCI-01-0145-FEDER-029841).Ventilator-associated pneumonia (VAP) is currently one of the top five most common nosocomial infections and it has been associated with prolonged hospitalization and high morbidity and mortality rates [1]. The use of an endotracheal tube (EET) constitutes the major risk factor for the development of this condition, since it is the airway access point for microorganisms. Indeed, microorganisms migration along and within the inside of the EET results in a so-called biofilm which, combined with a network of secretions, will be ultimately leaked down to lungs [2]. Biofilm formation is a crucial step in the pathogenesis of VAP, as microbial cells within a biofilm encase themselves in a self-produced matrix of extracellular polymeric substances, conferring them protection against both antimicrobial treatments and the host immune system. Furthermore, the polymicrobial nature of biofilms formed on the surfaces of ETT has been increasingly addressed [3]. This study aimed to design a wide-spectrum antimicrobial coating to impart endotracheal tubes with the ability to prevent the adhesion and subsequent biofilm formation of several microorganisms commonly associated to VAP, without compromising the viability of lung cells. For that, ciprofloxacin (CIP) and chlorhexidine (CHX), two compounds with different action spectra, were co-immobilized on polyvinyl chloride (PVC) surfaces to obtain a dual-drug release antimicrobial coating. Immobilization was performed using a one-step polydopamine (pDA)-based approach, in which PVC substrata were simply immersed on a solution of dopamine dissolved together with CHX and/or CIP, allowing their incorporation throughout the full thickness of the pDA film. The antimicrobial performance of modified surfaces was evaluated against 5 bacterial species (Pseudomonas aeruginosa, Acinetobacter baumannii, Klebsiella pneumoniae, Staphylococcus aureus and Staphylococcus epidermidis) and 1 yeast species (Candida albicans) as individual, dual and triple consortia. Regarding the single-species colonisation of PVC surfaces, results showed that after 24 h of exposure, co-immobilization of CHX and CIP caused a significant reduction (more than 4 Log) in the number of bacterial cells adhered to the surfaces, while being less efficient against the adhesion of C. albicans (0.5 Log). It is worth mentioning that the combination of CIP and CHX in the proposed coating resulted both in a synergic effect against P. aeruginosa, A. baumannii and C. albicans and in a facilitative effect against all tested species. Coatings were also tested against the dual adhesion of P. aeruginosa in consortium with all tested species. In general, results showed that the antimicrobial effect observed for each species was not compromised by the presence of any other species. Inhibition of C. albicans was actually more effective (approximately 2 Log reduction) when combined with P. aeruginosa. When the antimicrobial coating was exposed to a triple consortium comprised by P. aeruginosa, S. aureus and C. albicans, its antimicrobial effect against bacterial species was not compromised and it was able to inhibit the adhesion of yeast by about 1 Log. In terms of biocompatibility, the amount of CHX and CIP released from the surfaces did not compromise the growth of lung epithelial cells. Surfaces functionalization with CIP and/or CHX was confirmed by SEM, AFM and contact angle measurements. In conclusion, the combination of CIP and CHX, using dopamine chemistry, results in a coating with antimicrobial activity towards a wide spectrum of microorganisms commonly associated to VAP. Therefore, this approach holds great potential to be applied in EET, proving to be important in the fight against VAP. References [1] D. Nora, P. Póvoa, Antibiotic consumption and ventilator-associated pneumonia rates, some parallelism but some discrepancies, Ann. Transl. Med. (2017). doi:10.21037/atm.2017.09.16. [2] A. Rouzé, I. Martin-Loeches, S. Nseir, Airway Devices in Ventilator-Associated Pneumonia Pathogenesis and Prevention, Clin. Chest Med. (2018). doi:10.1016/j.ccm.2018.08.001. [3] M.E. Rodrigues, S.P. Lopes, C.R. Pereira, N.F. Azevedo, A. Lourenço, M. Henriques, M.O. Pereira, Polymicrobial ventilator-associated pneumonia: Fighting in vitro Candida albicans-pseudomonas aeruginosa biofilms with antifungal-antibacterial combination therapy, PLoS One. 12 (2017). doi:10.1371/journal.pone.0170433.info:eu-repo/semantics/publishedVersionUniversidade do MinhoAlves, Diana Filipa BarrosPereira, Maria OlíviaLopes, Susana Patrícia2021-09-072021-09-07T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/75660engAlves, Diana; Pereira, Maria Olívia; Lopes, Susana P., A wide-spectrum antimicrobial coating to fight multi-species biofilms in ventilator-associated pneumonia infections. ESB 2021 - 31st Annual Conference of the European Society for Biomaterials. Porto, Portugal, Sep 5-9, 2021.https://esb2021.org/info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T07:04:45Zoai:repositorium.sdum.uminho.pt:1822/75660Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:14:47.175969Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv A wide-spectrum antimicrobial coating to fight multi-species biofilms in ventilator-associated pneumonia infections
title A wide-spectrum antimicrobial coating to fight multi-species biofilms in ventilator-associated pneumonia infections
spellingShingle A wide-spectrum antimicrobial coating to fight multi-species biofilms in ventilator-associated pneumonia infections
Alves, Diana Filipa Barros
dual-drug release coating
ventilator-associated pneumonia
multi-species biofilms
title_short A wide-spectrum antimicrobial coating to fight multi-species biofilms in ventilator-associated pneumonia infections
title_full A wide-spectrum antimicrobial coating to fight multi-species biofilms in ventilator-associated pneumonia infections
title_fullStr A wide-spectrum antimicrobial coating to fight multi-species biofilms in ventilator-associated pneumonia infections
title_full_unstemmed A wide-spectrum antimicrobial coating to fight multi-species biofilms in ventilator-associated pneumonia infections
title_sort A wide-spectrum antimicrobial coating to fight multi-species biofilms in ventilator-associated pneumonia infections
author Alves, Diana Filipa Barros
author_facet Alves, Diana Filipa Barros
Pereira, Maria Olívia
Lopes, Susana Patrícia
author_role author
author2 Pereira, Maria Olívia
Lopes, Susana Patrícia
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Alves, Diana Filipa Barros
Pereira, Maria Olívia
Lopes, Susana Patrícia
dc.subject.por.fl_str_mv dual-drug release coating
ventilator-associated pneumonia
multi-species biofilms
topic dual-drug release coating
ventilator-associated pneumonia
multi-species biofilms
description This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. The authors also acknowledge the support, through the Programa Operacional Competitividade e Internacionalização (COMPETE2020) and by national funds, through the Portuguese Foundation for Science and Technology (FCT), of the POLY-PrevEnTT project (PTDC/BTM-SAL/29841/2017-POCI-01-0145-FEDER-029841).
publishDate 2021
dc.date.none.fl_str_mv 2021-09-07
2021-09-07T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/75660
url http://hdl.handle.net/1822/75660
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Alves, Diana; Pereira, Maria Olívia; Lopes, Susana P., A wide-spectrum antimicrobial coating to fight multi-species biofilms in ventilator-associated pneumonia infections. ESB 2021 - 31st Annual Conference of the European Society for Biomaterials. Porto, Portugal, Sep 5-9, 2021.
https://esb2021.org/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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