Evaluation the best condition of Fibrinolytic protease production using factorial design by Streptomyces sp DPUA 1573
| Main Author: | |
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| Publication Date: | 2012 |
| Other Authors: | , , , , , , , |
| Language: | eng |
| Source: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| Download full: | http://hdl.handle.net/1822/47926 |
Summary: | XI Reunião Regional Nordeste da SBBq | 4th International Symposium in Biochemistry of Macromolecules and Biotechnology |
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Evaluation the best condition of Fibrinolytic protease production using factorial design by Streptomyces sp DPUA 1573StreptomycesFibrinolytic enzymeIntravascular thrombosisXI Reunião Regional Nordeste da SBBq | 4th International Symposium in Biochemistry of Macromolecules and BiotechnologyFibrinolytic enzymes have the ability to degrade fibrin clots formed for avoiding intravascular thrombosis. In the pharmaceutical industry there is a search for new fibrinolytic agent that reduces the production cost and increasing productivity. The use of microorganism for enzyme production, such as the genus Streptomyces has been reported. Streptomyces is a Gram-positive bacteria, responsible for producing many bioactive compounds and extracellular enzymes of pharmaceutical interest. This study aimed to evaluated the production of fibrinolytic protease by Streptomyces sp DPUA 1573. Microbial cells were cultivated in the ISP2 for 48 hours, after this period the strains were inoculated in MS2 (soybean medium) that according with factorial design 24 (concentrations of soybean 0.5; 1.0 and 1.5%, glucose 0; 0.5 and 1.0% and different speeds 150 rpm; 200 rpm and 250 rpm and temperature 28C; 30C and 32C). The factorial design was analyzed by variance analysis (anova) and the glucose concentration showed a positive and significative effect. The results showed that the variable interaction had significant effect. that the best condition was composed 1.5% soybean, 1% glucose, 28 ºC and 150 speed in 48 hours, with production fibrinolytic 1391.66 U/mL. These values were higher than those reported in the literature. However these results show the biggest potencial in production fibrinolytic enzyme by Streptomyces.info:eu-repo/semantics/publishedVersionUniversidade do MinhoSilva, J. M.Clementino, E. L.Souza, K. P. S.Sales, Amanda EmmanuelleNascimento, Thiago PajeúTeixeira, J. A.Porto, Tatiana SouzaPorto, Ana Lúcia FigueiredoPorto, Camila Souza2012-12-052012-12-05T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/47926engSilva, J. M.; Clementino, E. L.; Souza, K. P. S.; Sales, A. E.; Nascimento, T. P.; Teixeira, José A.; Porto, T. S.; Porto, A. L. F.; Porto, C. S., Evaluation the best condition of Fibrinolytic protease production using factorial design by Streptomyces sp DPUA 1573. XI Reunião Regional Nordeste da SBBq | 4th International Symposium in Biochemistry of Macromolecules and Biotechnology. Recife, Brazil, Dec 5-7, 2012.http://www.sbbq.org.br/v2/index.php?option=com_content&task=view&id=746info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T05:48:32Zoai:repositorium.sdum.uminho.pt:1822/47926Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:30:41.996918Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
| dc.title.none.fl_str_mv |
Evaluation the best condition of Fibrinolytic protease production using factorial design by Streptomyces sp DPUA 1573 |
| title |
Evaluation the best condition of Fibrinolytic protease production using factorial design by Streptomyces sp DPUA 1573 |
| spellingShingle |
Evaluation the best condition of Fibrinolytic protease production using factorial design by Streptomyces sp DPUA 1573 Silva, J. M. Streptomyces Fibrinolytic enzyme Intravascular thrombosis |
| title_short |
Evaluation the best condition of Fibrinolytic protease production using factorial design by Streptomyces sp DPUA 1573 |
| title_full |
Evaluation the best condition of Fibrinolytic protease production using factorial design by Streptomyces sp DPUA 1573 |
| title_fullStr |
Evaluation the best condition of Fibrinolytic protease production using factorial design by Streptomyces sp DPUA 1573 |
| title_full_unstemmed |
Evaluation the best condition of Fibrinolytic protease production using factorial design by Streptomyces sp DPUA 1573 |
| title_sort |
Evaluation the best condition of Fibrinolytic protease production using factorial design by Streptomyces sp DPUA 1573 |
| author |
Silva, J. M. |
| author_facet |
Silva, J. M. Clementino, E. L. Souza, K. P. S. Sales, Amanda Emmanuelle Nascimento, Thiago Pajeú Teixeira, J. A. Porto, Tatiana Souza Porto, Ana Lúcia Figueiredo Porto, Camila Souza |
| author_role |
author |
| author2 |
Clementino, E. L. Souza, K. P. S. Sales, Amanda Emmanuelle Nascimento, Thiago Pajeú Teixeira, J. A. Porto, Tatiana Souza Porto, Ana Lúcia Figueiredo Porto, Camila Souza |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Silva, J. M. Clementino, E. L. Souza, K. P. S. Sales, Amanda Emmanuelle Nascimento, Thiago Pajeú Teixeira, J. A. Porto, Tatiana Souza Porto, Ana Lúcia Figueiredo Porto, Camila Souza |
| dc.subject.por.fl_str_mv |
Streptomyces Fibrinolytic enzyme Intravascular thrombosis |
| topic |
Streptomyces Fibrinolytic enzyme Intravascular thrombosis |
| description |
XI Reunião Regional Nordeste da SBBq | 4th International Symposium in Biochemistry of Macromolecules and Biotechnology |
| publishDate |
2012 |
| dc.date.none.fl_str_mv |
2012-12-05 2012-12-05T00:00:00Z |
| dc.type.driver.fl_str_mv |
conference object |
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info:eu-repo/semantics/publishedVersion |
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publishedVersion |
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http://hdl.handle.net/1822/47926 |
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http://hdl.handle.net/1822/47926 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Silva, J. M.; Clementino, E. L.; Souza, K. P. S.; Sales, A. E.; Nascimento, T. P.; Teixeira, José A.; Porto, T. S.; Porto, A. L. F.; Porto, C. S., Evaluation the best condition of Fibrinolytic protease production using factorial design by Streptomyces sp DPUA 1573. XI Reunião Regional Nordeste da SBBq | 4th International Symposium in Biochemistry of Macromolecules and Biotechnology. Recife, Brazil, Dec 5-7, 2012. http://www.sbbq.org.br/v2/index.php?option=com_content&task=view&id=746 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia |
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