Evidence that the degree of band 3 phosphorylation modulates human erythrocytes nitric oxide efflux – in vitro model of hyperfibrinogenemia

Bibliographic Details
Main Author: Almeida, J. P. Lopes de
Publication Date: 2011
Other Authors: Freitas-Santos, T., Saldanha, C.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10451/6574
Summary: © 2011 – IOS Press and the authors. All rights reserved
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spelling Evidence that the degree of band 3 phosphorylation modulates human erythrocytes nitric oxide efflux – in vitro model of hyperfibrinogenemiaBand 3 proteinErythrocyteFibrinogenNitric oxideNitratesNitrites© 2011 – IOS Press and the authors. All rights reservedRecent evidence has shown that plasma fibrinogen, a major cardiovascular risk factor, interacts with the erythrocyte membrane and acts to influence blood flow via erythrocyte nitric oxide (NO) modulation. In the present pioneer in-vitro study, whole blood samples were harvested from healthy subjects and aliquots were incubated in the absence (control aliquots) and presence of fibrinogen at different degrees of band 3 phosphorylation, and the levels of NO, nitrite, nitrate and S-nitroglutathione (GSNO) were determined. Hyperfibrinogenemia interferes with erythrocyte NO mobilization without changing its efflux in a way that seems to be dependent of the degree of band 3 phosphorylation. In presence of higher fibrinogen concentrations the NO efflux is reinforced when band 3 is phosphorylated (p < 0.001). Higher levels of nitrite, nitrate and GSNO were documented (p < 0.05). However, the mechanisms by which fibrinogen signalling modulates erythrocyte function remain to be clarified and are currently under study. These conditions may be considered an approach to be followed in blood storage for transfusions.This study was supported by grants from the FCT - Fundação para a Ciência e a Tecnologia (project reference PTDC/SAU-OSM/73449/2006)IOS PressRepositório da Universidade de LisboaAlmeida, J. P. Lopes deFreitas-Santos, T.Saldanha, C.2012-06-22T13:37:06Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/6574engClinical Hemorheology and Microcirculation 49 (2011) 407–4161386-0291info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T12:53:40Zoai:repositorio.ulisboa.pt:10451/6574Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T02:30:19.860812Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Evidence that the degree of band 3 phosphorylation modulates human erythrocytes nitric oxide efflux – in vitro model of hyperfibrinogenemia
title Evidence that the degree of band 3 phosphorylation modulates human erythrocytes nitric oxide efflux – in vitro model of hyperfibrinogenemia
spellingShingle Evidence that the degree of band 3 phosphorylation modulates human erythrocytes nitric oxide efflux – in vitro model of hyperfibrinogenemia
Almeida, J. P. Lopes de
Band 3 protein
Erythrocyte
Fibrinogen
Nitric oxide
Nitrates
Nitrites
title_short Evidence that the degree of band 3 phosphorylation modulates human erythrocytes nitric oxide efflux – in vitro model of hyperfibrinogenemia
title_full Evidence that the degree of band 3 phosphorylation modulates human erythrocytes nitric oxide efflux – in vitro model of hyperfibrinogenemia
title_fullStr Evidence that the degree of band 3 phosphorylation modulates human erythrocytes nitric oxide efflux – in vitro model of hyperfibrinogenemia
title_full_unstemmed Evidence that the degree of band 3 phosphorylation modulates human erythrocytes nitric oxide efflux – in vitro model of hyperfibrinogenemia
title_sort Evidence that the degree of band 3 phosphorylation modulates human erythrocytes nitric oxide efflux – in vitro model of hyperfibrinogenemia
author Almeida, J. P. Lopes de
author_facet Almeida, J. P. Lopes de
Freitas-Santos, T.
Saldanha, C.
author_role author
author2 Freitas-Santos, T.
Saldanha, C.
author2_role author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Almeida, J. P. Lopes de
Freitas-Santos, T.
Saldanha, C.
dc.subject.por.fl_str_mv Band 3 protein
Erythrocyte
Fibrinogen
Nitric oxide
Nitrates
Nitrites
topic Band 3 protein
Erythrocyte
Fibrinogen
Nitric oxide
Nitrates
Nitrites
description © 2011 – IOS Press and the authors. All rights reserved
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
2012-06-22T13:37:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/6574
url http://hdl.handle.net/10451/6574
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinical Hemorheology and Microcirculation 49 (2011) 407–416
1386-0291
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv IOS Press
publisher.none.fl_str_mv IOS Press
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833601387257135104

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