Nanostructured SBA-15 silica as an adjuvant in immunizations with hepatitis B vaccine

• Main Author: Scaramuzzi,Karina
• Publication Date: 2011
• Other Authors: Oliveira,Denise Cristina André, Carvalho,Luciana Vieira, Tambourgi,Denise Vilarinho, Tenório,Elisabeth Christina Nunes, Rizzi,Marisa, Mussalem,Juliana, Fantini,Márcia Carvalho de Abreu, Botosso,Viviane Fongaro, Sant´Anna,Osvaldo Augusto
• Format: Article
• Language: eng
• Source: Einstein (São Paulo)
• Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082011000400436
• topic: Hepatitis B; Oral vaccination; Adjuvants; Immunological memory

ABSTRACT Objective: To evaluate the applicability of SBA-15 silica as an adjuvant in immunizations with purified particles of the viral protein HBsAg, the main component of hepatitis B vaccine, Butang®, produced by Instituto Butantan. Methods: BALB/c mice orally or subcutaneously received 0.5 μg of HBsAg adsorbed/encapsulated to SBA-15 or adsorbed to Al(OH)3. To assess the secondary immune response, a subcutaneous booster was administered 30 days after the first immunization. Individual serum and fecal samples of each group were periodically collected for specific antibody titration by ELISA. Results: Analysis of secretory IgA showed that mice orally primed with HBsAg on SBA-15 had increased levels of specific antibodies in primary and secondary immune responses. Specific serum IgA and IgG titers in HBsAg:SBA-15-orally immunized mice reached higher levels after the booster, demonstrating the effectiveness of oral vaccination with the use of silica. All immunized groups showed higher IgG1 levels. Conclusion: Our results clearly indicate the promising use of SBA-15 as an adjuvant, especially in oral immunizations.