Comparative study of methods to aflatoxin M1 determination

Bibliographic Details
Main Author: Maria de Sylos, Célia
Publication Date: 1996
Other Authors: Rodriguez Amaya, Delia
Format: Article
Language: por
Source: Revista do Instituto Adolfo Lutz (Online)
Download full: https://periodicos.saude.sp.gov.br/RIAL/article/view/36587
Summary: Various trials were carried out to choose the extracting solvent and the best technique for removing interfering substances from samples of rnilk for the TLC detennination of aflatoxin Mj. For extraction acetone, methanol and chloroform were utilized either singly, in combination with each other or with water. Precipitation with heavy metal salts, partition between immiscible solvents and conventional column chromatography were evaluated for clean-up. Heavy metals by themselves were not effective in eliminating interfering substances. The combined use of chloroform and a chromatographic colurnn proved to be the most efficient procedure for extraction and c1ean-up. Colurnns packed with silica or silica-Cj8 gave better results than that of cellulose. HPLC with solid phase extraction, c1ean-up in silica gel column and trifluoroacetic acid derivatization and fluorescence detection presented much higher sensitivity and specificity than TLC with visual quantification based on fluorescence intensity.
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spelling Comparative study of methods to aflatoxin M1 determinationEstudo comparativo de métodos para determinação de aflatoxina M1Aflatoxin M1milkthin layer chromatographyhigh performance liquid chromatographyanalysisAflatoxina M1leiteCromatografia em camada delgadaCromatografia líquida de alta eficiênciaAnáliseVarious trials were carried out to choose the extracting solvent and the best technique for removing interfering substances from samples of rnilk for the TLC detennination of aflatoxin Mj. For extraction acetone, methanol and chloroform were utilized either singly, in combination with each other or with water. Precipitation with heavy metal salts, partition between immiscible solvents and conventional column chromatography were evaluated for clean-up. Heavy metals by themselves were not effective in eliminating interfering substances. The combined use of chloroform and a chromatographic colurnn proved to be the most efficient procedure for extraction and c1ean-up. Colurnns packed with silica or silica-Cj8 gave better results than that of cellulose. HPLC with solid phase extraction, c1ean-up in silica gel column and trifluoroacetic acid derivatization and fluorescence detection presented much higher sensitivity and specificity than TLC with visual quantification based on fluorescence intensity.Várias tentativas foram realizadas para escolher o solvente extratante mais eficiente e a melhor técnica para remover os interferentes de amostras de leite para a determinação de aflatoxina M1 em camada delgada. Na extração foram utilizados acetona, metanol e clorofórmio individualmente, misturados entre si ou com água. Precipitação com sais de metais pesados, partição entre sol ventes imiscíveis e colunas cromatográficas foram avaliados para promover a limpeza do extrato. A utilização de metais pesados não foi eficiente para eliminar os interferentes. O uso conjunto de clorofórmio e urna coluna cromatográfica demonstrou ser a maneira mais eficiente de extrair a aflatoxina M1 e remover substâncias interferentes. Colunas cromatográficas de sílica e sílica-C18 apresentaram melhores resultados que a coluna de celulose. A cromatografia líquida de alta eficiência com extração em fase sólida, limpeza em coluna de sílica gel e derivação da aflatoxina M1 por ácido trifluoroacético e detecção por fluorescência apresentou sensibilidade e especificidade bem maiores que o método por cromatografia em camada delgada com quantificação visual baseada na intensidade de fluorescência.Instituto Adolfo Lutz1996-06-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado pelos paresapplication/pdfhttps://periodicos.saude.sp.gov.br/RIAL/article/view/3658710.53393/rial.1996.56.36587Instituto Adolfo Lutz Journal - RIAL; Vol. 56 No. 1 (1996); 87-97Revista del Instituto Adolfo Lutz - RIAL; Vol. 56 Núm. 1 (1996); 87-97Revista do Instituto Adolfo Lutz; v. 56 n. 1 (1996); 87-971983-38140073-9855reponame:Revista do Instituto Adolfo Lutz (Online)instname:Instituto Adolfo Lutzinstacron:IALporhttps://periodicos.saude.sp.gov.br/RIAL/article/view/36587/34857Copyright (c) 1996 Célia Maria de Sylos, Delia Rodriguez Amayainfo:eu-repo/semantics/openAccessMaria de Sylos, Célia Rodriguez Amaya, Delia2024-02-21T17:53:09Zoai:ojs.periodicos.saude.sp.gov.br:article/36587Revistahttps://periodicos.saude.sp.gov.br/RIAL/indexPUBhttps://periodicos.saude.sp.gov.br/RIAL/oairial@saude.sp.gov.brhttps://doi.org/10.53393/rial1983-38140073-9855opendoar:2024-02-21T17:53:09Revista do Instituto Adolfo Lutz (Online) - Instituto Adolfo Lutzfalse
dc.title.none.fl_str_mv Comparative study of methods to aflatoxin M1 determination
Estudo comparativo de métodos para determinação de aflatoxina M1
title Comparative study of methods to aflatoxin M1 determination
spellingShingle Comparative study of methods to aflatoxin M1 determination
Maria de Sylos, Célia
Aflatoxin M1
milk
thin layer chromatography
high performance liquid chromatography
analysis
Aflatoxina M1
leite
Cromatografia em camada delgada
Cromatografia líquida de alta eficiência
Análise
title_short Comparative study of methods to aflatoxin M1 determination
title_full Comparative study of methods to aflatoxin M1 determination
title_fullStr Comparative study of methods to aflatoxin M1 determination
title_full_unstemmed Comparative study of methods to aflatoxin M1 determination
title_sort Comparative study of methods to aflatoxin M1 determination
author Maria de Sylos, Célia
author_facet Maria de Sylos, Célia
Rodriguez Amaya, Delia
author_role author
author2 Rodriguez Amaya, Delia
author2_role author
dc.contributor.author.fl_str_mv Maria de Sylos, Célia
Rodriguez Amaya, Delia
dc.subject.por.fl_str_mv Aflatoxin M1
milk
thin layer chromatography
high performance liquid chromatography
analysis
Aflatoxina M1
leite
Cromatografia em camada delgada
Cromatografia líquida de alta eficiência
Análise
topic Aflatoxin M1
milk
thin layer chromatography
high performance liquid chromatography
analysis
Aflatoxina M1
leite
Cromatografia em camada delgada
Cromatografia líquida de alta eficiência
Análise
description Various trials were carried out to choose the extracting solvent and the best technique for removing interfering substances from samples of rnilk for the TLC detennination of aflatoxin Mj. For extraction acetone, methanol and chloroform were utilized either singly, in combination with each other or with water. Precipitation with heavy metal salts, partition between immiscible solvents and conventional column chromatography were evaluated for clean-up. Heavy metals by themselves were not effective in eliminating interfering substances. The combined use of chloroform and a chromatographic colurnn proved to be the most efficient procedure for extraction and c1ean-up. Colurnns packed with silica or silica-Cj8 gave better results than that of cellulose. HPLC with solid phase extraction, c1ean-up in silica gel column and trifluoroacetic acid derivatization and fluorescence detection presented much higher sensitivity and specificity than TLC with visual quantification based on fluorescence intensity.
publishDate 1996
dc.date.none.fl_str_mv 1996-06-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.saude.sp.gov.br/RIAL/article/view/36587
10.53393/rial.1996.56.36587
url https://periodicos.saude.sp.gov.br/RIAL/article/view/36587
identifier_str_mv 10.53393/rial.1996.56.36587
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.saude.sp.gov.br/RIAL/article/view/36587/34857
dc.rights.driver.fl_str_mv Copyright (c) 1996 Célia Maria de Sylos, Delia Rodriguez Amaya
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 1996 Célia Maria de Sylos, Delia Rodriguez Amaya
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Adolfo Lutz
publisher.none.fl_str_mv Instituto Adolfo Lutz
dc.source.none.fl_str_mv Instituto Adolfo Lutz Journal - RIAL; Vol. 56 No. 1 (1996); 87-97
Revista del Instituto Adolfo Lutz - RIAL; Vol. 56 Núm. 1 (1996); 87-97
Revista do Instituto Adolfo Lutz; v. 56 n. 1 (1996); 87-97
1983-3814
0073-9855
reponame:Revista do Instituto Adolfo Lutz (Online)
instname:Instituto Adolfo Lutz
instacron:IAL
instname_str Instituto Adolfo Lutz
instacron_str IAL
institution IAL
reponame_str Revista do Instituto Adolfo Lutz (Online)
collection Revista do Instituto Adolfo Lutz (Online)
repository.name.fl_str_mv Revista do Instituto Adolfo Lutz (Online) - Instituto Adolfo Lutz
repository.mail.fl_str_mv rial@saude.sp.gov.br
_version_ 1836991963832778752

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