Occurrence of aflatoxin M1 in bovine milk samples consumed in different regions of Brazil

Bibliographic Details
Main Author: Shundo, Luzia
Publication Date: 2016
Other Authors: Almeida, Adriana Palma de, Alaburda, Janete, Lamardo, Leda Conceição Antonia, Navas, Sandra Aparecida, Ruvieri, Valter, Sabino, Myrna
Format: Article
Language: eng
Source: Revista do Instituto Adolfo Lutz (Online)
Download full: https://periodicos.saude.sp.gov.br/RIAL/article/view/33517
Summary: Two hundred and fifty-seven samples of milk proceeding from different geographical regions of Brazil were analyzed for determining the presence of aflatoxin M1 (AFM1). The AFM1 extraction was carried out using immunoaffinity column, separated by reversed-phase (C-18) high performance liquid chromatography (HPLC), and quantified by fluorescence detector. The Limits of Quantification (LOQ) were 0.008 μg/kg and 0.080 μg/kg to the fluid and the powder milk, respectively. AFM1 were detected in 209 (81.3 %) samples, being 26 (63.4 %), 105 (84.0 %) and 78 (85.7 %) of pasteurized, UHT (Ultra-high Temperature) and powder milk, respectively. The highest concentration of AFM1 in powder milk was found in one sample from Minas Gerais (1.210 μg/kg). In UHT and pasteurized milk, the highest levels were detected in one sample from Sergipe (0.120 μg/kg) and one sample from Goiás (0.050 μg/kg), respectively. None of the samples analyzed in this study exceeded the Brazilian legal limits for AFM1.
id IAL_a35a6539a19d4091a40f2528e32660c6
oai_identifier_str oai:ojs.periodicos.saude.sp.gov.br:article/33517
network_acronym_str IAL
network_name_str Revista do Instituto Adolfo Lutz (Online)
repository_id_str
spelling Occurrence of aflatoxin M1 in bovine milk samples consumed in different regions of BrazilOcorrência de aflatoxina M1 em amostras de leite bovino consumido em diferentes regiões do Brasilmycotoxinsimmunoaffinity columnhigh performance liquid chromatographyexposure assessmentmicotoxinascoluna de imunoafinidadecromatografia líquida de alta eficiênciaestimativa de exposiçãoTwo hundred and fifty-seven samples of milk proceeding from different geographical regions of Brazil were analyzed for determining the presence of aflatoxin M1 (AFM1). The AFM1 extraction was carried out using immunoaffinity column, separated by reversed-phase (C-18) high performance liquid chromatography (HPLC), and quantified by fluorescence detector. The Limits of Quantification (LOQ) were 0.008 μg/kg and 0.080 μg/kg to the fluid and the powder milk, respectively. AFM1 were detected in 209 (81.3 %) samples, being 26 (63.4 %), 105 (84.0 %) and 78 (85.7 %) of pasteurized, UHT (Ultra-high Temperature) and powder milk, respectively. The highest concentration of AFM1 in powder milk was found in one sample from Minas Gerais (1.210 μg/kg). In UHT and pasteurized milk, the highest levels were detected in one sample from Sergipe (0.120 μg/kg) and one sample from Goiás (0.050 μg/kg), respectively. None of the samples analyzed in this study exceeded the Brazilian legal limits for AFM1.Duzentas e cinquenta e sete amostras de leite provenientes das diferentes regiões geográficas do Brasil foram analisadas para realizar a determinação de aflatoxina M1 (AFM1). As AFM1 foram extraídas por meio de colunas de imunoafinidade, separadas por cromatografia líquida de alta eficiência em fase reversa (C-18) e quantificadas por detector de fluorescência (CLAE-FL). Os limites de quantificação (LQ) foram de 0,008 μg/kg e 0,080 μg/kg para o leite fluido e em pó, respectivamente. AFM1 foi detectada em 209 (81,3 %) amostras, sendo 26 (63,4 %), 105 (84,0 %) e 78 (85,7 %) para o leite pasteurizado, UHT (Ultra-high Temperature) e em pó, respectivamente. A maior concentração de AFM1 no leite em pó foi encontrada em uma amostra proveniente de Minas Gerais (1,210 μg/kg). No leite UHT e pasteurizado, os maiores níveis foram encontrados em uma amostra de Sergipe (0,120 μg/kg) e Goiás (0,050 μg/kg), respectivamente. Nenhuma amostra analisada ultrapassou os limites da legislação brasileira em vigor para AFM1.Instituto Adolfo Lutz2016-10-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado pelos paresapplication/pdfhttps://periodicos.saude.sp.gov.br/RIAL/article/view/3351710.53393/rial.2016.v75.33517Instituto Adolfo Lutz Journal - RIAL; Vol. 75 (2016); 01-08Revista del Instituto Adolfo Lutz - RIAL; Vol. 75 (2016); 01-08Revista do Instituto Adolfo Lutz; v. 75 (2016); 01-081983-38140073-9855reponame:Revista do Instituto Adolfo Lutz (Online)instname:Instituto Adolfo Lutzinstacron:IALenghttps://periodicos.saude.sp.gov.br/RIAL/article/view/33517/32349Copyright (c) 2016 Luzia Shundo, Adriana Palma de Almeida, Janete Alaburda, Leda Conceição Antonia Lamardo, Sandra Aparecida Navas, Valter Ruvieri, Myrna Sabinoinfo:eu-repo/semantics/openAccessShundo, LuziaAlmeida, Adriana Palma deAlaburda, JaneteLamardo, Leda Conceição AntoniaNavas, Sandra AparecidaRuvieri, ValterSabino, Myrna2024-07-04T18:11:50Zoai:ojs.periodicos.saude.sp.gov.br:article/33517Revistahttps://periodicos.saude.sp.gov.br/RIAL/indexPUBhttps://periodicos.saude.sp.gov.br/RIAL/oairial@saude.sp.gov.brhttps://doi.org/10.53393/rial1983-38140073-9855opendoar:2024-07-04T18:11:50Revista do Instituto Adolfo Lutz (Online) - Instituto Adolfo Lutzfalse
dc.title.none.fl_str_mv Occurrence of aflatoxin M1 in bovine milk samples consumed in different regions of Brazil
Ocorrência de aflatoxina M1 em amostras de leite bovino consumido em diferentes regiões do Brasil
title Occurrence of aflatoxin M1 in bovine milk samples consumed in different regions of Brazil
spellingShingle Occurrence of aflatoxin M1 in bovine milk samples consumed in different regions of Brazil
Shundo, Luzia
mycotoxins
immunoaffinity column
high performance liquid chromatography
exposure assessment
micotoxinas
coluna de imunoafinidade
cromatografia líquida de alta eficiência
estimativa de exposição
title_short Occurrence of aflatoxin M1 in bovine milk samples consumed in different regions of Brazil
title_full Occurrence of aflatoxin M1 in bovine milk samples consumed in different regions of Brazil
title_fullStr Occurrence of aflatoxin M1 in bovine milk samples consumed in different regions of Brazil
title_full_unstemmed Occurrence of aflatoxin M1 in bovine milk samples consumed in different regions of Brazil
title_sort Occurrence of aflatoxin M1 in bovine milk samples consumed in different regions of Brazil
author Shundo, Luzia
author_facet Shundo, Luzia
Almeida, Adriana Palma de
Alaburda, Janete
Lamardo, Leda Conceição Antonia
Navas, Sandra Aparecida
Ruvieri, Valter
Sabino, Myrna
author_role author
author2 Almeida, Adriana Palma de
Alaburda, Janete
Lamardo, Leda Conceição Antonia
Navas, Sandra Aparecida
Ruvieri, Valter
Sabino, Myrna
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Shundo, Luzia
Almeida, Adriana Palma de
Alaburda, Janete
Lamardo, Leda Conceição Antonia
Navas, Sandra Aparecida
Ruvieri, Valter
Sabino, Myrna
dc.subject.por.fl_str_mv mycotoxins
immunoaffinity column
high performance liquid chromatography
exposure assessment
micotoxinas
coluna de imunoafinidade
cromatografia líquida de alta eficiência
estimativa de exposição
topic mycotoxins
immunoaffinity column
high performance liquid chromatography
exposure assessment
micotoxinas
coluna de imunoafinidade
cromatografia líquida de alta eficiência
estimativa de exposição
description Two hundred and fifty-seven samples of milk proceeding from different geographical regions of Brazil were analyzed for determining the presence of aflatoxin M1 (AFM1). The AFM1 extraction was carried out using immunoaffinity column, separated by reversed-phase (C-18) high performance liquid chromatography (HPLC), and quantified by fluorescence detector. The Limits of Quantification (LOQ) were 0.008 μg/kg and 0.080 μg/kg to the fluid and the powder milk, respectively. AFM1 were detected in 209 (81.3 %) samples, being 26 (63.4 %), 105 (84.0 %) and 78 (85.7 %) of pasteurized, UHT (Ultra-high Temperature) and powder milk, respectively. The highest concentration of AFM1 in powder milk was found in one sample from Minas Gerais (1.210 μg/kg). In UHT and pasteurized milk, the highest levels were detected in one sample from Sergipe (0.120 μg/kg) and one sample from Goiás (0.050 μg/kg), respectively. None of the samples analyzed in this study exceeded the Brazilian legal limits for AFM1.
publishDate 2016
dc.date.none.fl_str_mv 2016-10-25
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.saude.sp.gov.br/RIAL/article/view/33517
10.53393/rial.2016.v75.33517
url https://periodicos.saude.sp.gov.br/RIAL/article/view/33517
identifier_str_mv 10.53393/rial.2016.v75.33517
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.saude.sp.gov.br/RIAL/article/view/33517/32349
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Adolfo Lutz
publisher.none.fl_str_mv Instituto Adolfo Lutz
dc.source.none.fl_str_mv Instituto Adolfo Lutz Journal - RIAL; Vol. 75 (2016); 01-08
Revista del Instituto Adolfo Lutz - RIAL; Vol. 75 (2016); 01-08
Revista do Instituto Adolfo Lutz; v. 75 (2016); 01-08
1983-3814
0073-9855
reponame:Revista do Instituto Adolfo Lutz (Online)
instname:Instituto Adolfo Lutz
instacron:IAL
instname_str Instituto Adolfo Lutz
instacron_str IAL
institution IAL
reponame_str Revista do Instituto Adolfo Lutz (Online)
collection Revista do Instituto Adolfo Lutz (Online)
repository.name.fl_str_mv Revista do Instituto Adolfo Lutz (Online) - Instituto Adolfo Lutz
repository.mail.fl_str_mv rial@saude.sp.gov.br
_version_ 1836991961562611712

Similar Items