Peroxidase - antiperoxidase unlabelled antibody technique (PAP) for the detection of hepatitis B surface antigen in hepatic tissue

Detalhes bibliográficos
Autor(a) principal: Alves, Venâncio Avancini Ferreira
Data de Publicação: 1980
Outros Autores: Morais, Carlos Floriano de, Santos, Raimunda Telma de Macedo, Takeda, Augusta Kiyomi, Gayotto, Luís Carlos da Costa
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista do Instituto Adolfo Lutz (Online)
Texto Completo: https://periodicos.saude.sp.gov.br/RIAL/article/view/36976
Resumo: Peroxidase - antiperoxidase unlabelled antibody technique onhepatic tissue sections was carried out for detection of hepatitis B surface antigen. This method demonstrated advantages over immunofluorescence, regardíng to its utilization in fixed 01' in block tissue, in routíne procedures, in optical or electronic microscopy, for visuaJization of detailed morphological tissue structures by selective staining and preparation of permanent records. This technique using ali of the immunological materiaIs prepared in our laboratory at Instituto Adolfo Lutz, São Paulo, Brazil, is an impor tan t step for its suitabiJity for use in diagnostic procedures.
id IAL_9425be1e4c88b4ac899a31a52ebd13f5
oai_identifier_str oai:ojs.periodicos.saude.sp.gov.br:article/36976
network_acronym_str IAL
network_name_str Revista do Instituto Adolfo Lutz (Online)
repository_id_str
spelling Peroxidase - antiperoxidase unlabelled antibody technique (PAP) for the detection of hepatitis B surface antigen in hepatic tissueUtilização da técnica de anticorpos não marcados peroxidase (PAP) na detecção do antígeno de superfície da hepatite B em tecido hepáticostaining, immunocitochemical technique for hepatic tissueantigens, surface, hepatitis Bhepatitis B surface antigensAustralia antigenscoloração de tecido hepático, técnica imunocitoquímicaantígenos de superfície da hepatite Bhepatite B, antígenosantígeno AustráliaPeroxidase - antiperoxidase unlabelled antibody technique onhepatic tissue sections was carried out for detection of hepatitis B surface antigen. This method demonstrated advantages over immunofluorescence, regardíng to its utilization in fixed 01' in block tissue, in routíne procedures, in optical or electronic microscopy, for visuaJization of detailed morphological tissue structures by selective staining and preparation of permanent records. This technique using ali of the immunological materiaIs prepared in our laboratory at Instituto Adolfo Lutz, São Paulo, Brazil, is an impor tan t step for its suitabiJity for use in diagnostic procedures.Foi utilizada a técnica de anticorpos não marcados peroxidase-antiperoxidase em fragmentos de tecido hepático para a detecção de antígeno de superfície da hepatite B. Em confronto com a imunofluorescência, esta reação apresenta as vantagens de aplicabilidade em tecidos fixados e emblocados rotineiramente, análise à microscopia óptica ou eletrônica, visualização de detalhes morfológicos do tecido por contracorantes, além de manutenção da reação por um tempo indefenido. A utilização, neste estudo, de arsenal imunológico totalmente produzido nos laboratórios do Instituto Adolfo Lutz, São Paulo, Brasil, é um importante pa ss o para a aplicabilidade deste método na rotina diagnóstica, em nosso meio.Instituto Adolfo Lutz1980-12-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado pelos paresapplication/pdfhttps://periodicos.saude.sp.gov.br/RIAL/article/view/3697610.53393/rial.1980.40.36976Instituto Adolfo Lutz Journal - RIAL; Vol. 40 No. 2 (1980); 101-106Revista del Instituto Adolfo Lutz - RIAL; Vol. 40 Núm. 2 (1980); 101-106Revista do Instituto Adolfo Lutz; v. 40 n. 2 (1980); 101-1061983-38140073-9855reponame:Revista do Instituto Adolfo Lutz (Online)instname:Instituto Adolfo Lutzinstacron:IALporhttps://periodicos.saude.sp.gov.br/RIAL/article/view/36976/35178Copyright (c) 1980 Venâncio Avancini Ferreira Alves, Carlos Floriano de Morais, Raimunda Telma de Macedo Santos, Augusta Kiyomi Takeda, Luís Carlos da Costa Gayottoinfo:eu-repo/semantics/openAccessAlves, Venâncio Avancini FerreiraMorais, Carlos Floriano deSantos, Raimunda Telma de MacedoTakeda, Augusta KiyomiGayotto, Luís Carlos da Costa2024-02-29T19:32:17Zoai:ojs.periodicos.saude.sp.gov.br:article/36976Revistahttps://periodicos.saude.sp.gov.br/RIAL/indexPUBhttps://periodicos.saude.sp.gov.br/RIAL/oairial@saude.sp.gov.brhttps://doi.org/10.53393/rial1983-38140073-9855opendoar:2024-02-29T19:32:17Revista do Instituto Adolfo Lutz (Online) - Instituto Adolfo Lutzfalse
dc.title.none.fl_str_mv Peroxidase - antiperoxidase unlabelled antibody technique (PAP) for the detection of hepatitis B surface antigen in hepatic tissue
Utilização da técnica de anticorpos não marcados peroxidase (PAP) na detecção do antígeno de superfície da hepatite B em tecido hepático
title Peroxidase - antiperoxidase unlabelled antibody technique (PAP) for the detection of hepatitis B surface antigen in hepatic tissue
spellingShingle Peroxidase - antiperoxidase unlabelled antibody technique (PAP) for the detection of hepatitis B surface antigen in hepatic tissue
Alves, Venâncio Avancini Ferreira
staining, immunocitochemical technique for hepatic tissue
antigens, surface, hepatitis B
hepatitis B surface antigens
Australia antigens
coloração de tecido hepático, técnica imunocitoquímica
antígenos de superfície da hepatite B
hepatite B, antígenos
antígeno Austrália
title_short Peroxidase - antiperoxidase unlabelled antibody technique (PAP) for the detection of hepatitis B surface antigen in hepatic tissue
title_full Peroxidase - antiperoxidase unlabelled antibody technique (PAP) for the detection of hepatitis B surface antigen in hepatic tissue
title_fullStr Peroxidase - antiperoxidase unlabelled antibody technique (PAP) for the detection of hepatitis B surface antigen in hepatic tissue
title_full_unstemmed Peroxidase - antiperoxidase unlabelled antibody technique (PAP) for the detection of hepatitis B surface antigen in hepatic tissue
title_sort Peroxidase - antiperoxidase unlabelled antibody technique (PAP) for the detection of hepatitis B surface antigen in hepatic tissue
author Alves, Venâncio Avancini Ferreira
author_facet Alves, Venâncio Avancini Ferreira
Morais, Carlos Floriano de
Santos, Raimunda Telma de Macedo
Takeda, Augusta Kiyomi
Gayotto, Luís Carlos da Costa
author_role author
author2 Morais, Carlos Floriano de
Santos, Raimunda Telma de Macedo
Takeda, Augusta Kiyomi
Gayotto, Luís Carlos da Costa
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Alves, Venâncio Avancini Ferreira
Morais, Carlos Floriano de
Santos, Raimunda Telma de Macedo
Takeda, Augusta Kiyomi
Gayotto, Luís Carlos da Costa
dc.subject.por.fl_str_mv staining, immunocitochemical technique for hepatic tissue
antigens, surface, hepatitis B
hepatitis B surface antigens
Australia antigens
coloração de tecido hepático, técnica imunocitoquímica
antígenos de superfície da hepatite B
hepatite B, antígenos
antígeno Austrália
topic staining, immunocitochemical technique for hepatic tissue
antigens, surface, hepatitis B
hepatitis B surface antigens
Australia antigens
coloração de tecido hepático, técnica imunocitoquímica
antígenos de superfície da hepatite B
hepatite B, antígenos
antígeno Austrália
description Peroxidase - antiperoxidase unlabelled antibody technique onhepatic tissue sections was carried out for detection of hepatitis B surface antigen. This method demonstrated advantages over immunofluorescence, regardíng to its utilization in fixed 01' in block tissue, in routíne procedures, in optical or electronic microscopy, for visuaJization of detailed morphological tissue structures by selective staining and preparation of permanent records. This technique using ali of the immunological materiaIs prepared in our laboratory at Instituto Adolfo Lutz, São Paulo, Brazil, is an impor tan t step for its suitabiJity for use in diagnostic procedures.
publishDate 1980
dc.date.none.fl_str_mv 1980-12-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.saude.sp.gov.br/RIAL/article/view/36976
10.53393/rial.1980.40.36976
url https://periodicos.saude.sp.gov.br/RIAL/article/view/36976
identifier_str_mv 10.53393/rial.1980.40.36976
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.saude.sp.gov.br/RIAL/article/view/36976/35178
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Adolfo Lutz
publisher.none.fl_str_mv Instituto Adolfo Lutz
dc.source.none.fl_str_mv Instituto Adolfo Lutz Journal - RIAL; Vol. 40 No. 2 (1980); 101-106
Revista del Instituto Adolfo Lutz - RIAL; Vol. 40 Núm. 2 (1980); 101-106
Revista do Instituto Adolfo Lutz; v. 40 n. 2 (1980); 101-106
1983-3814
0073-9855
reponame:Revista do Instituto Adolfo Lutz (Online)
instname:Instituto Adolfo Lutz
instacron:IAL
instname_str Instituto Adolfo Lutz
instacron_str IAL
institution IAL
reponame_str Revista do Instituto Adolfo Lutz (Online)
collection Revista do Instituto Adolfo Lutz (Online)
repository.name.fl_str_mv Revista do Instituto Adolfo Lutz (Online) - Instituto Adolfo Lutz
repository.mail.fl_str_mv rial@saude.sp.gov.br
_version_ 1836991965147693056

Registros relacionados