Recommendations for specimen collection for detection and diagnosis of COVID-19

Detalhes bibliográficos
Autor(a) principal: Dall'Agnol, Juliana
Data de Publicação: 2021
Outros Autores: Schwartz, Eda, Lise, Fernanda
Tipo de documento: Artigo
Idioma: eng
Título da fonte: ABCS Health Sciences (Online)
Texto Completo: https://www.portalnepas.org.br/abcshs/article/view/1642
Resumo: Introduction: The spread of SARS-CoV-2 has caused a global public health crisis (pandemic). One of the most important measures to control the transmission chain of the new coronavirus is to identify those infected through laboratory testing. Objective: Synthesize the recommendations for the specimen collection for detection and diagnosis of COVID-19. Methods: This is an integrative review, considering the publications of the following databases: PubMed and Google Academic from January 2020. Results: 468 publications were identified, 20 of which were considered eligible. The publications recommend that technical training for specimen collection and careful observation of infection prevention protocols are fundamental. This manuscript highlights the steps for specimen collection as materials for collection, storage, transportation, individual protection, and laboratory analysis of samples. Currently, the Reverse Transcription - Polymerase Chain Reaction test is the recommended and gold standard method of identifying COVID-19 cases. Serological tests play an important role in research and surveillance. Conclusion: In summary, the documents ensure that the RT-PCR is the gold standard for SARS-CoV-2 detection and recommend standardization of collection and conditioning methods to avoid errors related to the collection and false negative results.
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spelling Recommendations for specimen collection for detection and diagnosis of COVID-19Recomendações para a realização de coleta de amostras para detecção e diagnóstico de COVID-19specimen handlingcoronavirus infectionsprotein coronapandemicsdiagnosiscoleta de amostrasinfecções por coronavíruscoroa de proteínapandemiasdiagnósticoIntroduction: The spread of SARS-CoV-2 has caused a global public health crisis (pandemic). One of the most important measures to control the transmission chain of the new coronavirus is to identify those infected through laboratory testing. Objective: Synthesize the recommendations for the specimen collection for detection and diagnosis of COVID-19. Methods: This is an integrative review, considering the publications of the following databases: PubMed and Google Academic from January 2020. Results: 468 publications were identified, 20 of which were considered eligible. The publications recommend that technical training for specimen collection and careful observation of infection prevention protocols are fundamental. This manuscript highlights the steps for specimen collection as materials for collection, storage, transportation, individual protection, and laboratory analysis of samples. Currently, the Reverse Transcription - Polymerase Chain Reaction test is the recommended and gold standard method of identifying COVID-19 cases. Serological tests play an important role in research and surveillance. Conclusion: In summary, the documents ensure that the RT-PCR is the gold standard for SARS-CoV-2 detection and recommend standardization of collection and conditioning methods to avoid errors related to the collection and false negative results.Introdução: A disseminação do SARS-CoV-2 ocasionou uma crise na saúde pública mundial (pandemia). Uma das mais importantes medidas de controle da cadeia de transmissão do novo coronavírus consiste em identificar os infectados por meio de teste laboratorial. Objetivo: Sintetizar as recomendações para a coleta de amostras para detecção e diagnóstico da COVID-19. Método: Trata-se de uma revisão integrativa, considerando as publicações do Google Acadêmico e Pubmed a partir de janeiro de 2020. Resultados: Foram identificadas 468 publicações, das quais 20 foram consideradas elegíveis. As publicações recomendam que a capacitação técnica para a coleta das amostras e a observação criteriosa de protocolos de prevenção de infecção são fundamentais. Destacam-se nesse artigo as etapas para a coleta de amostras como materiais para coleta, armazenamento, transporte, proteção individual e análise laboratorial das amostras. Atualmente, o teste de Reverse Transcription - Polymerase Chain Reaction é o método recomendado e padrão-ouro para a identificação dos casos de COVID-19. Os testes sorológicos desempenham um papel importante na pesquisa e vigilância. Conclusão: Em síntese, os documentos asseguram que o RT-PCR é o teste padrão-ouro para detecção do SARS-CoV-2 e recomenda a padronização dos métodos de coleta e acondicionamento, a fim de evitar erros relacionados com a coleta e resultados falso-negativos.Núcleo de Estudos, Pesquisa e Assessoria à Saúde (NEPAS)2021-07-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado pelos paresapplication/pdfhttps://www.portalnepas.org.br/abcshs/article/view/164210.7322/abcshs.2020195.1642ABCS Health Sciences; Vol. 46 (2021); e021302ABCS Health Sciences; v. 46 (2021); e0213022357-81142318-4965reponame:ABCS Health Sciences (Online)instname:Faculdade de Medicina do ABC (FMABC)instacron:FMABCenghttps://www.portalnepas.org.br/abcshs/article/view/1642/1140Copyright (c) 2021 Dall’Agnol et al. info:eu-repo/semantics/openAccessDall'Agnol, JulianaSchwartz, EdaLise, Fernanda2021-12-24T22:52:13Zoai:ojs.emnuvens.com.br:article/1642Revistahttp://www.portalnepas.org.br/abcshsPUBhttp://www.portalnepas.org.br/abcshs/oairicardo.souto@fmabc.br2357-81142318-4965opendoar:2021-12-24T22:52:13ABCS Health Sciences (Online) - Faculdade de Medicina do ABC (FMABC)false
dc.title.none.fl_str_mv Recommendations for specimen collection for detection and diagnosis of COVID-19
Recomendações para a realização de coleta de amostras para detecção e diagnóstico de COVID-19
title Recommendations for specimen collection for detection and diagnosis of COVID-19
spellingShingle Recommendations for specimen collection for detection and diagnosis of COVID-19
Dall'Agnol, Juliana
specimen handling
coronavirus infections
protein corona
pandemics
diagnosis
coleta de amostras
infecções por coronavírus
coroa de proteína
pandemias
diagnóstico
title_short Recommendations for specimen collection for detection and diagnosis of COVID-19
title_full Recommendations for specimen collection for detection and diagnosis of COVID-19
title_fullStr Recommendations for specimen collection for detection and diagnosis of COVID-19
title_full_unstemmed Recommendations for specimen collection for detection and diagnosis of COVID-19
title_sort Recommendations for specimen collection for detection and diagnosis of COVID-19
author Dall'Agnol, Juliana
author_facet Dall'Agnol, Juliana
Schwartz, Eda
Lise, Fernanda
author_role author
author2 Schwartz, Eda
Lise, Fernanda
author2_role author
author
dc.contributor.author.fl_str_mv Dall'Agnol, Juliana
Schwartz, Eda
Lise, Fernanda
dc.subject.por.fl_str_mv specimen handling
coronavirus infections
protein corona
pandemics
diagnosis
coleta de amostras
infecções por coronavírus
coroa de proteína
pandemias
diagnóstico
topic specimen handling
coronavirus infections
protein corona
pandemics
diagnosis
coleta de amostras
infecções por coronavírus
coroa de proteína
pandemias
diagnóstico
description Introduction: The spread of SARS-CoV-2 has caused a global public health crisis (pandemic). One of the most important measures to control the transmission chain of the new coronavirus is to identify those infected through laboratory testing. Objective: Synthesize the recommendations for the specimen collection for detection and diagnosis of COVID-19. Methods: This is an integrative review, considering the publications of the following databases: PubMed and Google Academic from January 2020. Results: 468 publications were identified, 20 of which were considered eligible. The publications recommend that technical training for specimen collection and careful observation of infection prevention protocols are fundamental. This manuscript highlights the steps for specimen collection as materials for collection, storage, transportation, individual protection, and laboratory analysis of samples. Currently, the Reverse Transcription - Polymerase Chain Reaction test is the recommended and gold standard method of identifying COVID-19 cases. Serological tests play an important role in research and surveillance. Conclusion: In summary, the documents ensure that the RT-PCR is the gold standard for SARS-CoV-2 detection and recommend standardization of collection and conditioning methods to avoid errors related to the collection and false negative results.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-21
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.portalnepas.org.br/abcshs/article/view/1642
10.7322/abcshs.2020195.1642
url https://www.portalnepas.org.br/abcshs/article/view/1642
identifier_str_mv 10.7322/abcshs.2020195.1642
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.portalnepas.org.br/abcshs/article/view/1642/1140
dc.rights.driver.fl_str_mv Copyright (c) 2021 Dall’Agnol et al.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Dall’Agnol et al.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Núcleo de Estudos, Pesquisa e Assessoria à Saúde (NEPAS)
publisher.none.fl_str_mv Núcleo de Estudos, Pesquisa e Assessoria à Saúde (NEPAS)
dc.source.none.fl_str_mv ABCS Health Sciences; Vol. 46 (2021); e021302
ABCS Health Sciences; v. 46 (2021); e021302
2357-8114
2318-4965
reponame:ABCS Health Sciences (Online)
instname:Faculdade de Medicina do ABC (FMABC)
instacron:FMABC
instname_str Faculdade de Medicina do ABC (FMABC)
instacron_str FMABC
institution FMABC
reponame_str ABCS Health Sciences (Online)
collection ABCS Health Sciences (Online)
repository.name.fl_str_mv ABCS Health Sciences (Online) - Faculdade de Medicina do ABC (FMABC)
repository.mail.fl_str_mv ricardo.souto@fmabc.br
_version_ 1836732275612450817

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