T-cell receptor Vβ repertoire of CD8+ T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil
| Main Author: | |
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| Publication Date: | 2015 |
| Other Authors: | , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1590/0074-02760150039 |
| Download full: | https://arca.fiocruz.br/handle/icict/12216 |
Summary: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Núcleo de Purificação Celular (Sorting). Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil. |
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Ferraz, RaquelCunha, Clarissa FerreiraPimentel, Maria InêsLyra, Marcelo RosandiskiSchubach, Armando OliveiraMendonça, Sérgio Coutinho Furtado deCruz, Alda Maria daBertho, Álvaro Luiz2015-11-12T15:18:31Z2015-11-12T15:18:31Z2015FERRAZ, Raquel; et al. T-cell receptor Vβ repertoire of CD8+ T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil. Mem Inst Oswaldo Cruz, v.110, n.5, p.596-605, Aug. 2015.0074-0276https://arca.fiocruz.br/handle/icict/1221610.1590/0074-027601500391678-8060engFundação Oswaldo CruzT-cell receptor Vβ repertoire of CD8+ T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Núcleo de Purificação Celular (Sorting). Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância em Leishmaniose. Rio de Janeiro, RJ, Brasil.undação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância em Leishmaniose. Rio de Janeiro, RJ, Brasil.undação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância em Leishmaniose. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Núcleo de Purificação Celular (Sorting). Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil.In human cutaneous leishmaniasis (CL), the immune response is mainly mediated by T-cells. The role of CD8+ Tlymphocytes, which are related to healing or deleterious functions, in affecting clinical outcome is controversial. The aim of this study was to evaluate T-cell receptor diversity in late-differentiated effector (LDE) and memory CD8+ T-cell subsets in order to create a profile of specific clones engaged in deleterious or protective CL immune responses. Healthy subjects, patients with active disease (PAD) and clinically cured patients were enrolled in the study. Total CD8+ T-lymphocytes showed a disturbance in the expression of the Vβ2, Vβ9, Vβ13.2, Vβ18 and Vβ23 families. The analyses of CD8+ T-lymphocyte subsets showed high frequencies of LDE CD8+ T-lymphocytes expressing Vβ12 and Vβ22 in PAD, as well as effector-memory CD8+ T-cells expressing Vβ22. We also observed low frequencies of effector and central-memory CD8+ T-cells expressing Vβ2 in PAD, which correlated with a greater lesion size. Particular Vβ expansions point to CD8+ T-cell clones that are selected during CL immune responses, suggesting that CD8+ T-lymphocytes expressing Vβ12 or Vβ22 are involved in a LDE response and that Vβ2 contractions in memory CD8+ T-cells are associated with larger lesions.Vβ repertoireHuman cutaneous leishmaniasisFlow cytometryCD8+ T-lymphocyteLeishmania braziliensisTCRLeishmania braziliensisCitometria de FluxoLeishmaniose Cutâneainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/3f186dbd-e04d-4061-b425-ffde5a116bb6/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALraquel_ferraz_etal_IOC_2015.pdfraquel_ferraz_etal_IOC_2015.pdfapplication/pdf1196415https://arca.fiocruz.br/bitstreams/777385c3-be1a-476e-8724-b08c32293c54/download4b1187a1392fb54d4a9ba0de782eaa8eMD52trueAnonymousREADTEXTraquel_ferraz_etal_IOC_2015.pdf.txtraquel_ferraz_etal_IOC_2015.pdf.txtExtracted texttext/plain51117https://arca.fiocruz.br/bitstreams/765028dc-1c85-4e66-b987-5d5c2afd3a75/downloadd816c86b070f1d482870f0a5c1371902MD55falseAnonymousREADTHUMBNAILraquel_ferraz_etal_IOC_2015.pdf.jpgraquel_ferraz_etal_IOC_2015.pdf.jpgGenerated Thumbnailimage/jpeg6051https://arca.fiocruz.br/bitstreams/ffc4546f-b904-4d38-aa72-43cbd8101cb1/download87ad1038464a1bc7963e9f0bbe74f143MD56falseAnonymousREADicict/122162025-07-30 00:01:54.315open.accessoai:arca.fiocruz.br:icict/12216https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T03:01:54Repositório Institucional da FIOCRUZ (ARCA) - 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| dc.title.none.fl_str_mv |
T-cell receptor Vβ repertoire of CD8+ T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil |
| title |
T-cell receptor Vβ repertoire of CD8+ T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil |
| spellingShingle |
T-cell receptor Vβ repertoire of CD8+ T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil Ferraz, Raquel Vβ repertoire Human cutaneous leishmaniasis Flow cytometry CD8+ T-lymphocyte Leishmania braziliensis TCR Leishmania braziliensis Citometria de Fluxo Leishmaniose Cutânea |
| title_short |
T-cell receptor Vβ repertoire of CD8+ T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil |
| title_full |
T-cell receptor Vβ repertoire of CD8+ T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil |
| title_fullStr |
T-cell receptor Vβ repertoire of CD8+ T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil |
| title_full_unstemmed |
T-cell receptor Vβ repertoire of CD8+ T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil |
| title_sort |
T-cell receptor Vβ repertoire of CD8+ T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil |
| author |
Ferraz, Raquel |
| author_facet |
Ferraz, Raquel Cunha, Clarissa Ferreira Pimentel, Maria Inês Lyra, Marcelo Rosandiski Schubach, Armando Oliveira Mendonça, Sérgio Coutinho Furtado de Cruz, Alda Maria da Bertho, Álvaro Luiz |
| author_role |
author |
| author2 |
Cunha, Clarissa Ferreira Pimentel, Maria Inês Lyra, Marcelo Rosandiski Schubach, Armando Oliveira Mendonça, Sérgio Coutinho Furtado de Cruz, Alda Maria da Bertho, Álvaro Luiz |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Ferraz, Raquel Cunha, Clarissa Ferreira Pimentel, Maria Inês Lyra, Marcelo Rosandiski Schubach, Armando Oliveira Mendonça, Sérgio Coutinho Furtado de Cruz, Alda Maria da Bertho, Álvaro Luiz |
| dc.subject.en.none.fl_str_mv |
Vβ repertoire Human cutaneous leishmaniasis Flow cytometry CD8+ T-lymphocyte Leishmania braziliensis TCR |
| topic |
Vβ repertoire Human cutaneous leishmaniasis Flow cytometry CD8+ T-lymphocyte Leishmania braziliensis TCR Leishmania braziliensis Citometria de Fluxo Leishmaniose Cutânea |
| dc.subject.decs.none.fl_str_mv |
Leishmania braziliensis Citometria de Fluxo Leishmaniose Cutânea |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Núcleo de Purificação Celular (Sorting). Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil. |
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2015 |
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2015-11-12T15:18:31Z |
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2015-11-12T15:18:31Z |
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2015 |
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FERRAZ, Raquel; et al. T-cell receptor Vβ repertoire of CD8+ T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil. Mem Inst Oswaldo Cruz, v.110, n.5, p.596-605, Aug. 2015. |
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https://arca.fiocruz.br/handle/icict/12216 |
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0074-0276 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/0074-02760150039 |
| dc.identifier.eissn.none.fl_str_mv |
1678-8060 |
| identifier_str_mv |
FERRAZ, Raquel; et al. T-cell receptor Vβ repertoire of CD8+ T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil. Mem Inst Oswaldo Cruz, v.110, n.5, p.596-605, Aug. 2015. 0074-0276 10.1590/0074-02760150039 1678-8060 |
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Fundação Oswaldo Cruz |
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Fundação Oswaldo Cruz |
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