Evolution of kdr haplotypes in worldwide populations of Aedes aegypti: Independent origins of the F1534C kdr mutation

Bibliographic Details
Main Author: Cosme, Luciano Veiga
Publication Date: 2020
Other Authors: Gloria-Soria, Andrea, Caccone, Adalgisa, Powell, Jeffrey Robert, Martins, Ademir Jesus
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1371/journal.pntd.0008219
Download full: https://arca.fiocruz.br/handle/icict/41448
Summary: Yale University. New Haven, CT, USA.
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spelling Cosme, Luciano VeigaGloria-Soria, AndreaCaccone, AdalgisaPowell, Jeffrey RobertMartins, Ademir Jesus2020-05-29T14:08:19Z2020-05-29T14:08:19Z2020COSME, Luciano Veiga et al. Evolution of kdr haplotypes in worldwide populations of Aedes aegypti: independent origins of the F1534C kdr mutation. PLoS Neglected Tropical Diseases, v. 14, n. 4, p. 1-18, Apr. 2020.1935-2727https://arca.fiocruz.br/handle/icict/4144810.1371/journal.pntd.00082191935-2735engPublic Library of ScienceAedes aegyptiHaplótiposMutação kdrAedes aegyptiF1534C kdr mutationHaplotypesEvolution of kdr haplotypes in worldwide populations of Aedes aegypti: Independent origins of the F1534C kdr mutationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleYale University. New Haven, CT, USA.Yale University. New Haven, CT, USA / Center for Vector Biology & Zoonotic Diseases. The Connectivut Agricultural Experiment Station. New Haven, CT, USA.Yale University. New Haven, CT, USA.Yale University. New Haven, CT, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Aedes aegypti is the primary vector of dengue, chikungunya, Zika, and urban yellow fever. Insecticides are often the most effective tools to rapidly decrease the density of vector populations, especially during arbovirus disease outbreaks. However, the intense use of insecticides, particularly pyrethroids, has selected for resistant mosquito populations worldwide. Mutations in the voltage gated sodium channel (NaV) are among the principal mechanisms of resistance to pyrethroids and DDT, also known as "knockdown resistance," kdr. Here we report studies on the origin and dispersion of kdr haplotypes in samples of Ae. aegypti from its worldwide distribution. We amplified the IIS6 and IIIS6 NaV segments from pools of Ae. aegypti populations from 15 countries, in South and North America, Africa, Asia, Pacific, and Australia. The amplicons were barcoded and sequenced using NGS Ion Torrent. Output data were filtered and analyzed using the bioinformatic pipeline Seekdeep to determine frequencies of the IIS6 and IIIS6 haplotypes per population. Phylogenetic relationships among the haplotypes were used to infer whether the kdr mutations have a single or multiple origin. We found 26 and 18 haplotypes, respectively for the IIS6 and IIIS6 segments, among which were the known kdr mutations 989P, 1011M, 1016I and 1016G (IIS6), 1520I, and 1534C (IIIS6). The highest diversity of haplotypes was found in African samples. Kdr mutations 1011M and 1016I were found only in American and African populations, 989P + 1016G and 1520I + 1534C in Asia, while 1534C was present in samples from all continents, except Australia. Based primarily on the intron sequence, IIS6 haplotypes were subdivided into two well-defined clades (A and B). Subsequent phasing of the IIS6 + IIIS6 haplotypes indicates two distinct origins for the 1534C kdr mutation. These results provide evidence of kdr mutations arising de novo at specific locations within the Ae. aegypti geographic distribution. In addition, our results suggest that the 1534C kdr mutation had at least two independent origins. We can thus conclude that insecticide selection pressure with DDT and more recently with pyrethroids is selecting for independent convergent mutations in NaV.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/4115b474-6681-40c8-acd6-22e6c0880fd3/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALAdemirJesus_Martins_etal_IOC_2020.pdfAdemirJesus_Martins_etal_IOC_2020.pdfapplication/pdf2387339https://arca.fiocruz.br/bitstreams/26ea4809-f1e3-40d3-ba46-e29bdfb2143d/downloadf3c69c18466bc8da54c5f61279c35720MD52trueAnonymousREADTEXTAdemirJesus_Martins_etal_IOC_2020.pdf.txtAdemirJesus_Martins_etal_IOC_2020.pdf.txtExtracted texttext/plain71029https://arca.fiocruz.br/bitstreams/701f5c65-fdd3-40ac-afc8-580af5046c2c/download5e4f190e6d6fbf0382b883c4689b219dMD55falseAnonymousREADTHUMBNAILAdemirJesus_Martins_etal_IOC_2020.pdf.jpgAdemirJesus_Martins_etal_IOC_2020.pdf.jpgGenerated Thumbnailimage/jpeg5944https://arca.fiocruz.br/bitstreams/7df84c39-494c-4347-8584-e7a8408cc0c0/downloadd83a94e946166d364c16833330c60a31MD56falseAnonymousREADicict/414482025-07-31 13:24:35.816open.accessoai:arca.fiocruz.br:icict/41448https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-31T16:24:35Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.none.fl_str_mv Evolution of kdr haplotypes in worldwide populations of Aedes aegypti: Independent origins of the F1534C kdr mutation
title Evolution of kdr haplotypes in worldwide populations of Aedes aegypti: Independent origins of the F1534C kdr mutation
spellingShingle Evolution of kdr haplotypes in worldwide populations of Aedes aegypti: Independent origins of the F1534C kdr mutation
Cosme, Luciano Veiga
Aedes aegypti
Haplótipos
Mutação kdr
Aedes aegypti
F1534C kdr mutation
Haplotypes
title_short Evolution of kdr haplotypes in worldwide populations of Aedes aegypti: Independent origins of the F1534C kdr mutation
title_full Evolution of kdr haplotypes in worldwide populations of Aedes aegypti: Independent origins of the F1534C kdr mutation
title_fullStr Evolution of kdr haplotypes in worldwide populations of Aedes aegypti: Independent origins of the F1534C kdr mutation
title_full_unstemmed Evolution of kdr haplotypes in worldwide populations of Aedes aegypti: Independent origins of the F1534C kdr mutation
title_sort Evolution of kdr haplotypes in worldwide populations of Aedes aegypti: Independent origins of the F1534C kdr mutation
author Cosme, Luciano Veiga
author_facet Cosme, Luciano Veiga
Gloria-Soria, Andrea
Caccone, Adalgisa
Powell, Jeffrey Robert
Martins, Ademir Jesus
author_role author
author2 Gloria-Soria, Andrea
Caccone, Adalgisa
Powell, Jeffrey Robert
Martins, Ademir Jesus
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Cosme, Luciano Veiga
Gloria-Soria, Andrea
Caccone, Adalgisa
Powell, Jeffrey Robert
Martins, Ademir Jesus
dc.subject.other.none.fl_str_mv Aedes aegypti
Haplótipos
Mutação kdr
topic Aedes aegypti
Haplótipos
Mutação kdr
Aedes aegypti
F1534C kdr mutation
Haplotypes
dc.subject.en.none.fl_str_mv Aedes aegypti
F1534C kdr mutation
Haplotypes
description Yale University. New Haven, CT, USA.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-05-29T14:08:19Z
dc.date.available.fl_str_mv 2020-05-29T14:08:19Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COSME, Luciano Veiga et al. Evolution of kdr haplotypes in worldwide populations of Aedes aegypti: independent origins of the F1534C kdr mutation. PLoS Neglected Tropical Diseases, v. 14, n. 4, p. 1-18, Apr. 2020.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/41448
dc.identifier.issn.none.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0008219
dc.identifier.eissn.none.fl_str_mv 1935-2735
identifier_str_mv COSME, Luciano Veiga et al. Evolution of kdr haplotypes in worldwide populations of Aedes aegypti: independent origins of the F1534C kdr mutation. PLoS Neglected Tropical Diseases, v. 14, n. 4, p. 1-18, Apr. 2020.
1935-2727
10.1371/journal.pntd.0008219
1935-2735
url https://arca.fiocruz.br/handle/icict/41448
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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