Fibronectin modulates thymocyte-thymic epithelial cell interactions following Trypanosoma cruzi infection

Bibliographic Details
Main Author: Oliveira, Désio Aurélio Farias de
Publication Date: 2013
Other Authors: Almeida, Vinicius Cotta de, Vila-Verde, Déa Maria S., Riederer, Ingo, Meis, Juliana de, Savino, Wilson
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1590/0074-0276130071
Download full: https://arca.fiocruz.br/handle/icict/11750
Summary: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil.
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spelling Oliveira, Désio Aurélio Farias deAlmeida, Vinicius Cotta deVila-Verde, Déa Maria S.Riederer, IngoMeis, Juliana deSavino, Wilson2015-09-21T17:25:38Z2015-09-21T17:25:38Z2013OLIVEIRA, Désio Aurélio Farias de; et al. Fibronectin modulates thymocyte-thymic epithelial cell interactions following Trypanosoma cruzi infection. Mem Inst Oswaldo Cruz, v.108, n.7, p.825-831, Nov. 2013.1678-80600074-0276https://arca.fiocruz.br/handle/icict/1175010.1590/0074-0276130071engFundação Oswaldo CruzFibronectin modulates thymocyte-thymic epithelial cell interactions following Trypanosoma cruzi infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil.Developing thymocytes interact with thymic epithelial cells (TECs) through cell-cell interactions, TEC-derived secretory moieties and extracellular matrix (ECM)-mediated interactions. These physiological interactions are crucial for normal thymocyte differentiation, but can be disrupted in pathological situations. Indeed, there is severe thymic atrophy in animals acutely infected with Trypanosoma cruzi due to CD4+CD8+ thymocyte depletion secondary to caspase-mediated apoptosis, together with changes in ECM deposition and thymocyte migration. We studied an in vitro model of TEC infection by T. cruzi and found that infected TEC cultures show a reduced number of cells, which was likely associated with decreased proliferative capacity, but not with increased cell death, as demonstrated by bromodeoxyuridine and annexin-V labelling. The infected TEC cultures exhibited increased expression of fibronectin (FN), laminin (LM) and type IV collagen. Importantly, treatment with FN increased the relative number of infected cells, whereas treatment with anti-FN or anti-LM antibodies resulted in lower infection rates. Consistent with these data, we observed increased thymocyte adhesion to infected TEC cultures. Overall, these results suggest that ECM molecules, particularly FN, facilitate infection of the thymic epithelium and that the consequent enhancement of ECM expression might be associated with changes in TEC-thymocyte interactions.Trypanosoma cruziThymic epithelial cellsThymocytesFibronectinLamininTrypanosoma cruziLamininaFibronectinasTimócitosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://arca.fiocruz.br/bitstreams/ad787d53-7d60-4146-9cf3-cb1bf3132999/download7d48279ffeed55da8dfe2f8e81f3b81fMD51falseAnonymousREADORIGINALingo_riederer_etal_IOC-2013.pdfapplication/pdf908486https://arca.fiocruz.br/bitstreams/ae51540e-06ab-46ba-9270-28a9b206b149/downloadd191a27b32868ba636d701ed0ec19cfcMD52trueAnonymousREADTEXTingo_riederer_etal_IOC-2013.pdf.txtingo_riederer_etal_IOC-2013.pdf.txtExtracted texttext/plain35026https://arca.fiocruz.br/bitstreams/5f362912-f130-4639-927e-dde348a031e2/downloada835d47d8ac5f4f98e187ef9649a1e46MD55falseAnonymousREADTHUMBNAILingo_riederer_etal_IOC-2013.pdf.jpgingo_riederer_etal_IOC-2013.pdf.jpgGenerated Thumbnailimage/jpeg5873https://arca.fiocruz.br/bitstreams/2b498c9f-c530-4a03-ada3-e53a52b4699b/download4797859e9e668319789ebfeaf98a3ad7MD56falseAnonymousREADicict/117502025-07-29 23:02:37.853open.accessoai:arca.fiocruz.br:icict/11750https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T02:02:37Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)falseTElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4K
dc.title.none.fl_str_mv Fibronectin modulates thymocyte-thymic epithelial cell interactions following Trypanosoma cruzi infection
title Fibronectin modulates thymocyte-thymic epithelial cell interactions following Trypanosoma cruzi infection
spellingShingle Fibronectin modulates thymocyte-thymic epithelial cell interactions following Trypanosoma cruzi infection
Oliveira, Désio Aurélio Farias de
Trypanosoma cruzi
Thymic epithelial cells
Thymocytes
Fibronectin
Laminin
Trypanosoma cruzi
Laminina
Fibronectinas
Timócitos
title_short Fibronectin modulates thymocyte-thymic epithelial cell interactions following Trypanosoma cruzi infection
title_full Fibronectin modulates thymocyte-thymic epithelial cell interactions following Trypanosoma cruzi infection
title_fullStr Fibronectin modulates thymocyte-thymic epithelial cell interactions following Trypanosoma cruzi infection
title_full_unstemmed Fibronectin modulates thymocyte-thymic epithelial cell interactions following Trypanosoma cruzi infection
title_sort Fibronectin modulates thymocyte-thymic epithelial cell interactions following Trypanosoma cruzi infection
author Oliveira, Désio Aurélio Farias de
author_facet Oliveira, Désio Aurélio Farias de
Almeida, Vinicius Cotta de
Vila-Verde, Déa Maria S.
Riederer, Ingo
Meis, Juliana de
Savino, Wilson
author_role author
author2 Almeida, Vinicius Cotta de
Vila-Verde, Déa Maria S.
Riederer, Ingo
Meis, Juliana de
Savino, Wilson
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Désio Aurélio Farias de
Almeida, Vinicius Cotta de
Vila-Verde, Déa Maria S.
Riederer, Ingo
Meis, Juliana de
Savino, Wilson
dc.subject.en.none.fl_str_mv Trypanosoma cruzi
Thymic epithelial cells
Thymocytes
Fibronectin
Laminin
topic Trypanosoma cruzi
Thymic epithelial cells
Thymocytes
Fibronectin
Laminin
Trypanosoma cruzi
Laminina
Fibronectinas
Timócitos
dc.subject.decs.none.fl_str_mv Trypanosoma cruzi
Laminina
Fibronectinas
Timócitos
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-09-21T17:25:38Z
dc.date.available.fl_str_mv 2015-09-21T17:25:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Désio Aurélio Farias de; et al. Fibronectin modulates thymocyte-thymic epithelial cell interactions following Trypanosoma cruzi infection. Mem Inst Oswaldo Cruz, v.108, n.7, p.825-831, Nov. 2013.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/11750
dc.identifier.issn.none.fl_str_mv 1678-8060
0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-0276130071
identifier_str_mv OLIVEIRA, Désio Aurélio Farias de; et al. Fibronectin modulates thymocyte-thymic epithelial cell interactions following Trypanosoma cruzi infection. Mem Inst Oswaldo Cruz, v.108, n.7, p.825-831, Nov. 2013.
1678-8060
0074-0276
10.1590/0074-0276130071
url https://arca.fiocruz.br/handle/icict/11750
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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