Specific immunization of mice against Leishmania mexicana amazonensis using solubilized promastigotes

Bibliographic Details
Main Author: Barral Netto, Manoel
Publication Date: 1987
Other Authors: Reed, Steven G., Sadigursky, Moysés, Sonnenfeld, G.
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
Download full: https://arca.fiocruz.br/handle/icict/14757
Summary: Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Cornell University Medical College. New York, NY
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spelling Barral Netto, ManoelReed, Steven G.Sadigursky, MoysésSonnenfeld, G.2016-07-07T19:04:18Z2016-07-04T17:54:15Z1987BARRAL-NETTO, M. et al. Specific immunization of mice against Leishmania mexicana amazonensis using solubilized promastigotes. Clinical Experimental and Immunology, v. 67, p. 11-19, 1987.0009-9104https://arca.fiocruz.br/handle/icict/14757engWilleySpecific immunization of mice against Leishmania mexicana amazonensis using solubilized promastigotesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Cornell University Medical College. New York, NYFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Cornell University Medical College. New York, NYUniversity of Louisville. School of Medicine. Department of Microbiology and Immunology. Louisville, KYUniversity of Louisville. School of Medicine. Department of Microbiology and Immunology. Louisville, KYSuccessful immunization of highly susceptible BALB/c mice against progressive infection by Leishmania mexicana amazonensis, using whole solubilized promastigotes was achieved. The best immunization schedule consisted of three weekly injections of 5 x 107 parasite equivalents. Intravenous was superior to intraperitoneal or subcutaneous immunization. Protection persisted for up to 2 months after immunization, and beneficial effects could be observed in long-term follow-up (24 weeks after infection). Immunized mice exhibited marked reduction in primary lesion size, as well as reduction of the number of parasites in the spleen, and developed less metastases. High titres of specific anti-L. m. amazonensis IgG antibodies resulted from immunization, but titres did not correlate with protection. Groups with widely differing pre-infection antibody titres were equally protected, and similar antibody titres resulted in different levels of protection. Immunization alone did not induce significant serum interferon-gamma levels and specific delayedtype hypersensitivity (DTH) reactions, but resulted in the persistence of positive (DTH) reactions after infection, at a time when infected control animals had suppressed responses. Resistance to leishmaniasis appears to depend on cell mediated immune mechanisms, and the possibility of immunization with a solubilized antigen without adjuvant is intriguing and opens new perspectives in this area.LeishmaniasisVaccineBALB/c miceSolubilized antigens03 Saúde e Bem-Estarinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTBarral NM Specific immunization....pdf.txtBarral NM Specific immunization....pdf.txtExtracted texttext/plain28361https://arca.fiocruz.br/bitstreams/82ddf18e-0e3c-4a5d-82c8-7c4a07a12503/download5bd5b8bbf86574e7eb940188b115471dMD56falseAnonymousREADORIGINALBarral NM Specific immunization....pdfapplication/pdf978554https://arca.fiocruz.br/bitstreams/e6f5e474-2439-4ec2-bab1-4c17b33943aa/downloadad947959781bcceaa366c9cc6682513aMD52trueAnonymousREADLICENSElicense.txttext/plain2991https://arca.fiocruz.br/bitstreams/cbb62b3b-79c7-4e06-ba10-945771a0dd98/download5a560609d32a3863062d77ff32785d58MD53falseAnonymousREADTHUMBNAILBarral NM Specific immunization....pdf.jpgBarral NM Specific immunization....pdf.jpgGenerated Thumbnailimage/jpeg5644https://arca.fiocruz.br/bitstreams/754b630c-b8c9-44d4-b110-9acb1490d00e/downloadcda9a00378d1a86ae393ed15f41dda3fMD55falseAnonymousREADicict/147572025-08-03 15:46:37.565open.accessoai:arca.fiocruz.br:icict/14757https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-08-03T18:46:37Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.none.fl_str_mv Specific immunization of mice against Leishmania mexicana amazonensis using solubilized promastigotes
title Specific immunization of mice against Leishmania mexicana amazonensis using solubilized promastigotes
spellingShingle Specific immunization of mice against Leishmania mexicana amazonensis using solubilized promastigotes
Barral Netto, Manoel
Leishmaniasis
Vaccine
BALB/c mice
Solubilized antigens
03 Saúde e Bem-Estar
title_short Specific immunization of mice against Leishmania mexicana amazonensis using solubilized promastigotes
title_full Specific immunization of mice against Leishmania mexicana amazonensis using solubilized promastigotes
title_fullStr Specific immunization of mice against Leishmania mexicana amazonensis using solubilized promastigotes
title_full_unstemmed Specific immunization of mice against Leishmania mexicana amazonensis using solubilized promastigotes
title_sort Specific immunization of mice against Leishmania mexicana amazonensis using solubilized promastigotes
author Barral Netto, Manoel
author_facet Barral Netto, Manoel
Reed, Steven G.
Sadigursky, Moysés
Sonnenfeld, G.
author_role author
author2 Reed, Steven G.
Sadigursky, Moysés
Sonnenfeld, G.
author2_role author
author
author
dc.contributor.author.fl_str_mv Barral Netto, Manoel
Reed, Steven G.
Sadigursky, Moysés
Sonnenfeld, G.
dc.subject.en.none.fl_str_mv Leishmaniasis
Vaccine
BALB/c mice
Solubilized antigens
topic Leishmaniasis
Vaccine
BALB/c mice
Solubilized antigens
03 Saúde e Bem-Estar
dc.subject.ods.none.fl_str_mv 03 Saúde e Bem-Estar
description Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Cornell University Medical College. New York, NY
publishDate 1987
dc.date.issued.fl_str_mv 1987
dc.date.accessioned.fl_str_mv 2016-07-07T19:04:18Z
dc.date.available.fl_str_mv 2016-07-04T17:54:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BARRAL-NETTO, M. et al. Specific immunization of mice against Leishmania mexicana amazonensis using solubilized promastigotes. Clinical Experimental and Immunology, v. 67, p. 11-19, 1987.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/14757
dc.identifier.issn.none.fl_str_mv 0009-9104
identifier_str_mv BARRAL-NETTO, M. et al. Specific immunization of mice against Leishmania mexicana amazonensis using solubilized promastigotes. Clinical Experimental and Immunology, v. 67, p. 11-19, 1987.
0009-9104
url https://arca.fiocruz.br/handle/icict/14757
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Willey
publisher.none.fl_str_mv Willey
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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