Unveiling polysomal long non-coding RNA expression on the first day of adipogenesis and osteogenesis in human adipose-derived stem cells

Detalhes bibliográficos
Autor(a) principal: Bonilauri, Bernardo
Data de Publicação: 2024
Outros Autores: Ribeiro, Annanda Lyra, Spangenberg, Lucia, Dallagiovanna, Bruno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.3390/ijms25042013
Texto Completo: https://arca.fiocruz.br/handle/icict/67638
Resumo: This work was funded by CNPq PROEP/ICC Grant 442353/2019-7. B.B. received a fellowship from Fiocruz, and B.D received a fellowship from CNPq.
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spelling Bonilauri, BernardoRibeiro, Annanda LyraSpangenberg, LuciaDallagiovanna, Bruno2024-12-17T01:01:04Z2024-12-17T01:01:04Z2024BONILAURI, B. et al. Unveiling polysomal long non-coding RNA expression on the first day of adipogenesis and osteogenesis in human adipose-derived stem cells. International Journal of Molecular Sciences, v. 25, n. 2013, p. 1-17, 2024.1422-0067https://arca.fiocruz.br/handle/icict/6763810.3390/ijms25042013This work was funded by CNPq PROEP/ICC Grant 442353/2019-7. B.B. received a fellowship from Fiocruz, and B.D received a fellowship from CNPq.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células-tronco. Curitiba, PR, Brasil / Stanford University School of Medicine. Stanford Cardiovascular Institute. Stanford, USA.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células-tronco. Curitiba, PR, Brasil.Institut Pasteur de Montevideo. Bioinformatics Unit. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células-tronco. Curitiba, PR, Brasil.Understanding the intricate molecular mechanisms governing the fate of human adipose-derived stem cells (hASCs) is essential for elucidating the delicate balance between adipogenic and osteogenic differentiation in both healthy and pathological conditions. Long non-coding RNAs (lncR-NAs) have emerged as key regulators involved in lineage commitment and differentiation of stem cells, operating at various levels of gene regulation, including transcriptional, post-transcriptional, and post-translational processes. To gain deeper insights into the role of lncRNAs' in hASCs' differentiation, we conducted a comprehensive analysis of the lncRNA transcriptome (RNA-seq) and translatome (polysomal-RNA-seq) during a 24 h period of adipogenesis and osteogenesis. Our findings revealed distinct expression patterns between the transcriptome and translatome during both differentiation processes, highlighting 90 lncRNAs that are exclusively regulated in the polysomal fraction. These findings underscore the significance of investigating lncRNAs associated with ribosomes, considering their unique expression patterns and potential mechanisms of action, such as translational regulation and potential coding capacity for microproteins. Additionally, we identified specific lncRNA gene expression programs associated with adipogenesis and osteogenesis during the early stages of cell differentiation. By shedding light on the expression and potential functions of these polysome-associated lncRNAs, we aim to deepen our understanding of their involvement in the regulation of adipogenic and osteogenic differentiation, ultimately paving the way for novel therapeutic strategies and insights into regenerative medicine.engMDPIAdipogêneseCélulas troncoOsteogêneseDiferenciaçãoPolissomoTraduçãolncRNAsAdipogenesisOsteogenesisStem cellsDifferentiationPolysomeTranslationAdipogeniaCélulas-Tronco EmbrionáriasPolirribossomosUnveiling polysomal long non-coding RNA expression on the first day of adipogenesis and osteogenesis in human adipose-derived stem cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/4a5a4e7e-bd47-4788-ab46-39efb81fd5d7/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALve_Bernardo_Bonilauri_etal_ICC_2024.pdfve_Bernardo_Bonilauri_etal_ICC_2024.pdfapplication/pdf2670633https://arca.fiocruz.br/bitstreams/0b64dc36-f67d-4730-91d6-e5b2c5860e24/download04f681a817e0a5a746f465f374eba280MD52trueAnonymousREADTEXTve_Bernardo_Bonilauri_etal_ICC_2024.pdf.txtve_Bernardo_Bonilauri_etal_ICC_2024.pdf.txtExtracted texttext/plain72156https://arca.fiocruz.br/bitstreams/ad530573-78c0-4064-bcd1-1fcfd30c66c7/download00af223dc96e7b7ccab7377b8f42896bMD55falseAnonymousREADTHUMBNAILve_Bernardo_Bonilauri_etal_ICC_2024.pdf.jpgve_Bernardo_Bonilauri_etal_ICC_2024.pdf.jpgGenerated Thumbnailimage/jpeg5955https://arca.fiocruz.br/bitstreams/8fe7b0d7-32e4-41f2-821c-9f552082f539/downloadecec239258c35438e8e84dae9f88ecdeMD56falseAnonymousREADicict/676382025-07-29 22:17:53.275open.accessoai:arca.fiocruz.br:icict/67638https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T01:17:53Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.none.fl_str_mv Unveiling polysomal long non-coding RNA expression on the first day of adipogenesis and osteogenesis in human adipose-derived stem cells
title Unveiling polysomal long non-coding RNA expression on the first day of adipogenesis and osteogenesis in human adipose-derived stem cells
spellingShingle Unveiling polysomal long non-coding RNA expression on the first day of adipogenesis and osteogenesis in human adipose-derived stem cells
Bonilauri, Bernardo
Adipogênese
Células tronco
Osteogênese
Diferenciação
Polissomo
Tradução
lncRNAs
Adipogenesis
Osteogenesis
Stem cells
Differentiation
Polysome
Translation
Adipogenia
Células-Tronco Embrionárias
Polirribossomos
title_short Unveiling polysomal long non-coding RNA expression on the first day of adipogenesis and osteogenesis in human adipose-derived stem cells
title_full Unveiling polysomal long non-coding RNA expression on the first day of adipogenesis and osteogenesis in human adipose-derived stem cells
title_fullStr Unveiling polysomal long non-coding RNA expression on the first day of adipogenesis and osteogenesis in human adipose-derived stem cells
title_full_unstemmed Unveiling polysomal long non-coding RNA expression on the first day of adipogenesis and osteogenesis in human adipose-derived stem cells
title_sort Unveiling polysomal long non-coding RNA expression on the first day of adipogenesis and osteogenesis in human adipose-derived stem cells
author Bonilauri, Bernardo
author_facet Bonilauri, Bernardo
Ribeiro, Annanda Lyra
Spangenberg, Lucia
Dallagiovanna, Bruno
author_role author
author2 Ribeiro, Annanda Lyra
Spangenberg, Lucia
Dallagiovanna, Bruno
author2_role author
author
author
dc.contributor.author.fl_str_mv Bonilauri, Bernardo
Ribeiro, Annanda Lyra
Spangenberg, Lucia
Dallagiovanna, Bruno
dc.subject.other.none.fl_str_mv Adipogênese
Células tronco
Osteogênese
Diferenciação
Polissomo
Tradução
topic Adipogênese
Células tronco
Osteogênese
Diferenciação
Polissomo
Tradução
lncRNAs
Adipogenesis
Osteogenesis
Stem cells
Differentiation
Polysome
Translation
Adipogenia
Células-Tronco Embrionárias
Polirribossomos
dc.subject.en.none.fl_str_mv lncRNAs
Adipogenesis
Osteogenesis
Stem cells
Differentiation
Polysome
Translation
dc.subject.decs.none.fl_str_mv Adipogenia
Células-Tronco Embrionárias
Polirribossomos
description This work was funded by CNPq PROEP/ICC Grant 442353/2019-7. B.B. received a fellowship from Fiocruz, and B.D received a fellowship from CNPq.
publishDate 2024
dc.date.accessioned.fl_str_mv 2024-12-17T01:01:04Z
dc.date.available.fl_str_mv 2024-12-17T01:01:04Z
dc.date.issued.fl_str_mv 2024
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BONILAURI, B. et al. Unveiling polysomal long non-coding RNA expression on the first day of adipogenesis and osteogenesis in human adipose-derived stem cells. International Journal of Molecular Sciences, v. 25, n. 2013, p. 1-17, 2024.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/67638
dc.identifier.issn.none.fl_str_mv 1422-0067
dc.identifier.doi.none.fl_str_mv 10.3390/ijms25042013
identifier_str_mv BONILAURI, B. et al. Unveiling polysomal long non-coding RNA expression on the first day of adipogenesis and osteogenesis in human adipose-derived stem cells. International Journal of Molecular Sciences, v. 25, n. 2013, p. 1-17, 2024.
1422-0067
10.3390/ijms25042013
url https://arca.fiocruz.br/handle/icict/67638
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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