An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels

Bibliographic Details
Main Author: Gonçalves, Antonio M.
Publication Date: 1990
Other Authors: Nehme, Nedia S., Morel, Carlos Medicis
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1590/S0074-02761990000100017
Download full: https://arca.fiocruz.br/handle/icict/14608
Summary: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
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spelling Gonçalves, Antonio M.Nehme, Nedia S.Morel, Carlos Medicis2016-07-07T11:43:05Z2016-06-29T14:09:19Z1990GONCALVES, Antonio M.; NEHME, Nedia S.; MOREL, Carlos Medicis. An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels. Mem. Inst. Oswaldo Cruz [online], v.85, n.1, p. 101-106, 1990.1678-8060https://arca.fiocruz.br/handle/icict/1460810.1590/S0074-02761990000100017engFundação Oswaldo CruzAn improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gelsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.A simple protocol is described for the silver staining of polyacrylamide gradient gels used for the separation of restriction fragments of kinetoplast DNA [schizodeme analysis of trypanosomatids (Morel et al., 1980)]. The method overcomes the problems of non-uniform staining and strong background color which are frequently encountered when conventional protocols for silver staining of linear gels. The method described has proven to be of general applicability for DNA, RNA and protein separations in gradient gels.Silver stainingSchizodeme analysisDNARNAProteinTrypanosomatidsImpregnação pela PrataDNARNAProteínainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTcarlos13_morel_etal_IOC_1990.pdf.txtcarlos13_morel_etal_IOC_1990.pdf.txtExtracted texttext/plain12https://arca.fiocruz.br/bitstreams/5053c15b-3be2-4fc8-b307-e93b02a4a881/download36c6f0b2061da514c400c0bc2749b5cfMD56falseAnonymousREADORIGINALcarlos13_morel_etal_IOC_1990.pdfapplication/pdf224198https://arca.fiocruz.br/bitstreams/773b6793-0c38-4ea0-903a-66744ce5f9df/download8904d03bdc1774f03253273b913cf438MD52trueAnonymousREADLICENSElicense.txttext/plain2991https://arca.fiocruz.br/bitstreams/0f31aef6-0cea-4453-b087-c1fdc6fc564b/download5a560609d32a3863062d77ff32785d58MD53falseAnonymousREADTHUMBNAILcarlos13_morel_etal_IOC_1990.pdf.jpgcarlos13_morel_etal_IOC_1990.pdf.jpgGenerated Thumbnailimage/jpeg7413https://arca.fiocruz.br/bitstreams/6906f82d-4cda-4957-bd49-c5b0db2a0b97/downloadd1dbe768149c96dc1f6737467a6da450MD57falseAnonymousREADicict/146082025-07-29 23:08:27.373open.accessoai:arca.fiocruz.br:icict/14608https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T02:08:27Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.none.fl_str_mv An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels
title An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels
spellingShingle An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels
Gonçalves, Antonio M.
Silver staining
Schizodeme analysis
DNA
RNA
Protein
Trypanosomatids
Impregnação pela Prata
DNA
RNA
Proteína
title_short An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels
title_full An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels
title_fullStr An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels
title_full_unstemmed An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels
title_sort An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels
author Gonçalves, Antonio M.
author_facet Gonçalves, Antonio M.
Nehme, Nedia S.
Morel, Carlos Medicis
author_role author
author2 Nehme, Nedia S.
Morel, Carlos Medicis
author2_role author
author
dc.contributor.author.fl_str_mv Gonçalves, Antonio M.
Nehme, Nedia S.
Morel, Carlos Medicis
dc.subject.en.none.fl_str_mv Silver staining
Schizodeme analysis
DNA
RNA
Protein
Trypanosomatids
topic Silver staining
Schizodeme analysis
DNA
RNA
Protein
Trypanosomatids
Impregnação pela Prata
DNA
RNA
Proteína
dc.subject.decs.none.fl_str_mv Impregnação pela Prata
DNA
RNA
Proteína
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
publishDate 1990
dc.date.issued.fl_str_mv 1990
dc.date.accessioned.fl_str_mv 2016-07-07T11:43:05Z
dc.date.available.fl_str_mv 2016-06-29T14:09:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GONCALVES, Antonio M.; NEHME, Nedia S.; MOREL, Carlos Medicis. An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels. Mem. Inst. Oswaldo Cruz [online], v.85, n.1, p. 101-106, 1990.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/14608
dc.identifier.issn.none.fl_str_mv 1678-8060
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02761990000100017
identifier_str_mv GONCALVES, Antonio M.; NEHME, Nedia S.; MOREL, Carlos Medicis. An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels. Mem. Inst. Oswaldo Cruz [online], v.85, n.1, p. 101-106, 1990.
1678-8060
10.1590/S0074-02761990000100017
url https://arca.fiocruz.br/handle/icict/14608
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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