Viability of Human Adenovirus from Hospital Fomites

Bibliographic Details
Main Author: Ganime, Ana Carolina
Publication Date: 2014
Other Authors: Costa, Filipe A. Carvalho, Santos, Marisa, Costa Filho, Rubens, Leite, José Paulo G., Miagostovich, Marize P.
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1002/jmv.23907
Download full: https://arca.fiocruz.br/handle/icict/10813
Summary: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
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spelling Ganime, Ana CarolinaCosta, Filipe A. CarvalhoSantos, MarisaCosta Filho, RubensLeite, José Paulo G.Miagostovich, Marize P.2015-06-12T13:57:49Z2015-06-12T13:57:49Z2014GANIME, Ana Carolina et al. Viability of Human Adenovirus from Hospital Fomites. Journal of Medical Virology, v.86, n.2, p.2065-2069, dec. 2014.1096-9071https://arca.fiocruz.br/handle/icict/1081310.1002/jmv.23907engWiley Periodicals Inc.BiomarcadorAdenovirusViabilityFomitesHospitalBiomarkerFômitesAdenovirusViability of Human Adenovirus from Hospital Fomitesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Cardiologia. Rio de Janeiro, RJ, Brasil.Hospital Pró-Cardíaco. Rio de Janeiro, Rj, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.The monitoring of environmental microbial contamination in healthcare facilities may be a valuable tool to determine pathogens transmission in those settings; however, such procedure is limited to bacterial indicators. Viruses are found commonly in those environments and are rarely used for these procedures. The aim of this study was to assess distribution and viability of a human DNA virus on fomites in an Adult Intensive Care Unit of a private hospital in Rio de Janeiro, Brazil. Human adenoviruses (HAdV) were investigated in 141 fomites by scraping the surface area and screening by quantitative PCR (qPCR) using TaqMan® System (Carlsbad, CA). Ten positive samples were selected for virus isolation in A549 and/or HEp2c cell lines. A total of 63 samples (44.7%) were positive and presented viral load ranging from 2.48 × 101 to 2.1 × 103 genomic copies per millilitre (gc/ml). The viability was demonstrated by integrated cell culture/nested-PCR in 5 out of 10 samples. Nucleotide sequencing confirmed all samples as HAdV and characterized one of them as specie B, serotype 3 (HAdV-3). The results indicate the risk of nosocomial transmission via contaminated fomites and point out the use of HAdV as biomarkers of environmental contamination.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://arca.fiocruz.br/bitstreams/09c9a3fb-5ee7-40f4-b036-a3c0d8f1666f/download7d48279ffeed55da8dfe2f8e81f3b81fMD51falseAnonymousREADORIGINALana_ganimeetal_IOC_2014.pdfapplication/pdf74238https://arca.fiocruz.br/bitstreams/79f84498-39e7-473f-9e3e-d4191f98daa5/downloada4e579cacc9dcdada79fa6af28b95420MD52trueAnonymousREADTEXTana_ganimeetal_IOC_2014.pdf.txtana_ganimeetal_IOC_2014.pdf.txtExtracted texttext/plain28731https://arca.fiocruz.br/bitstreams/6622cf80-9961-40a6-8f19-dcdf47f65b49/download9382171f3c0d7875759cee30d416ee34MD55falseAnonymousREADTHUMBNAILana_ganimeetal_IOC_2014.pdf.jpgana_ganimeetal_IOC_2014.pdf.jpgGenerated Thumbnailimage/jpeg6263https://arca.fiocruz.br/bitstreams/e5fb2f02-fd55-4965-9dc6-227ca16a793f/download5577bd72b04e3beca1aa5f4cb7658aedMD56falseAnonymousREADicict/108132025-07-30 02:55:47.882open.accessoai:arca.fiocruz.br:icict/10813https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T05:55:47Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)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
dc.title.none.fl_str_mv Viability of Human Adenovirus from Hospital Fomites
title Viability of Human Adenovirus from Hospital Fomites
spellingShingle Viability of Human Adenovirus from Hospital Fomites
Ganime, Ana Carolina
Biomarcador
Adenovirus
Viability
Fomites
Hospital
Biomarker
Fômites
Adenovirus
title_short Viability of Human Adenovirus from Hospital Fomites
title_full Viability of Human Adenovirus from Hospital Fomites
title_fullStr Viability of Human Adenovirus from Hospital Fomites
title_full_unstemmed Viability of Human Adenovirus from Hospital Fomites
title_sort Viability of Human Adenovirus from Hospital Fomites
author Ganime, Ana Carolina
author_facet Ganime, Ana Carolina
Costa, Filipe A. Carvalho
Santos, Marisa
Costa Filho, Rubens
Leite, José Paulo G.
Miagostovich, Marize P.
author_role author
author2 Costa, Filipe A. Carvalho
Santos, Marisa
Costa Filho, Rubens
Leite, José Paulo G.
Miagostovich, Marize P.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Ganime, Ana Carolina
Costa, Filipe A. Carvalho
Santos, Marisa
Costa Filho, Rubens
Leite, José Paulo G.
Miagostovich, Marize P.
dc.subject.other.none.fl_str_mv Biomarcador
topic Biomarcador
Adenovirus
Viability
Fomites
Hospital
Biomarker
Fômites
Adenovirus
dc.subject.en.none.fl_str_mv Adenovirus
Viability
Fomites
Hospital
Biomarker
dc.subject.decs.none.fl_str_mv Fômites
Adenovirus
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-06-12T13:57:49Z
dc.date.available.fl_str_mv 2015-06-12T13:57:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GANIME, Ana Carolina et al. Viability of Human Adenovirus from Hospital Fomites. Journal of Medical Virology, v.86, n.2, p.2065-2069, dec. 2014.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/10813
dc.identifier.issn.none.fl_str_mv 1096-9071
dc.identifier.doi.none.fl_str_mv 10.1002/jmv.23907
identifier_str_mv GANIME, Ana Carolina et al. Viability of Human Adenovirus from Hospital Fomites. Journal of Medical Virology, v.86, n.2, p.2065-2069, dec. 2014.
1096-9071
10.1002/jmv.23907
url https://arca.fiocruz.br/handle/icict/10813
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley Periodicals Inc.
publisher.none.fl_str_mv Wiley Periodicals Inc.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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