Novel isothermal nucleic acid amplification method for detecting malaria parasites

Bibliographic Details
Main Author: Queiroz, Lucas Tavares de
Publication Date: 2024
Other Authors: Baptista, Barbara de Oliveira, Santos, Rebecca de Abreu Fernandes dos, Pereira, Carolina de Souza Faria, Lemos, Juliana Aline de Souza, Souza, Hugo Amorim dos Santos de, Martorano, Rodrigo Medeiros, Riccio, Evelyn Kety Pratt, Totino, Paulo Renato Rivas, Ferreira, Joseli de Oliveira, Lima Junior, Josué da Costa, Ribeiro, Cláudio Tadeu Daniel, Riccio, Lilian Rose Pratt
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1007/s00253-024-13357-2
Download full: https://arca.fiocruz.br/handle/icict/67839
Summary: Produção científica do Laboratório de Imunoparasitologia.
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spelling Queiroz, Lucas Tavares deBaptista, Barbara de OliveiraSantos, Rebecca de Abreu Fernandes dosPereira, Carolina de Souza FariaLemos, Juliana Aline de SouzaSouza, Hugo Amorim dos Santos deMartorano, Rodrigo MedeirosRiccio, Evelyn Kety PrattTotino, Paulo Renato RivasFerreira, Joseli de OliveiraLima Junior, Josué da CostaRibeiro, Cláudio Tadeu DanielRiccio, Lilian Rose Pratt2025-01-02T14:44:48Z2025-01-02T14:44:48Z2024QUEIROZ, Lucas Tavares de et al. Novel isothermal nucleic acid amplification method for detecting malaria parasites. Applied Microbiology and Biotechnology, v. 108, n. 544, p. 1-15, 27 Dec. 2024.0175-7598https://arca.fiocruz.br/handle/icict/6783910.1007/s00253-024-13357-21432-0614Produção científica do Laboratório de Imunoparasitologia.Produção científica do Laboratório de Pesquisa em Malária.This research was supported by Fundação Oswaldo Cruz (INOVA VPPCB-007-FIO-18–2-46), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 425.422/2016–0), Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (Pronex E-26/010.001268/2016), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES Finance Code 001).Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brasil.Universidade Federal do Acre. Instituto da Biodiversidade. Laboratório de Doenças Infecciosas da Amazônia Ocidental. Cruzeiro do Sul, AC, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brasil.Malaria, a parasitic disease caused by Plasmodium spp. and transmitted by Anopheles mosquitoes, remains a major global health issue, with an estimated 249 million cases and 608,000 deaths in 2022. Rapid and accurate diagnosis and treatment are crucial for malaria control and elimination. However, limited access to sensitive molecular tests means that microscopic examination and rapid diagnostic tests (RDT) are the most used methods in endemic areas, despite their lower diagnostic accuracy. Therefore, there is a need for developing sensitive, simple, accurate, and rapid diagnostic tools suitable for field conditions. Herein, we aimed to explore the potential of the enzymatic recombinase amplification assay (ERA® Technology) as a remote laboratory test by evaluating and validating the GENEYE® ERA Plasmodium detection kit in Brazilian endemic areas. A cross-sectional cohort study was conducted between June and August of 2023 in the Brazilian Amazon. The study enrolled 323 participants residing in three malaria-affected regions: Cruzeiro do Sul and Mâncio Lima (Acre State) and Guajará (Amazonas State). The participants were tested for malaria by microscopy, rapid diagnostic tests (RDT), nested PCR (nPCR), quantitative real-time PCR (qPCR), and ERA. The sensitivity, specificity, and predictive values were assessed using nPCR as a gold standard. Plasmodium prevalence was 21.7%, 18.8%, 19.2%, 21.7%, and 21.7% by nPCR, microscopy, RDT, qPCR, and ERA respectively. Using nPCR as the standard, qPCR, and ERA showed a sensitivity of 100%. In comparison, microscopy and RDT showed a sensitivity of 87.1% and 88.6%, a negative predictive value (NPV) of 96.56 and 96.93, and kappa values of 0.91 and 0.92, respectively. For Plasmodium falciparum, the sensitivity of qPCR and ERA was 100% while the sensitivity of microscopy and RDT was 96.9% and 93.7%, and the NPV was 99.66 and 99.32, respectively. For Plasmodium vivax, only ERA showed the same sensitivity of nPCR. The sensitivity, NPV, and kappa values were 78.85%, 97.27, and 0.87 for qPCR and microscopy, and 84.21%, 97.94, and 0.9 for RDT. The data presented here show that the GENEYE® ERA Plasmodium detection kit offers a promising alternative to traditional malaria diagnostic methods. Its high sensitivity, specificity, fast processing time, and operational simplicity position it as a valuable point-of-care diagnostic tool, particularly in resource-limited and remote malaria-endemic areas.engSpringerNovel isothermal nucleic acid amplification method for detecting malaria parasitesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlasmodium detectionIsothermal nucleic acid amplifcationRapid diagnostic testsPoint-of-careBrazilian AmazonMalaria03 Saúde e Bem-Estar09 Indústria, inovação e infraestrutura17 Parcerias e meios de implementaçãoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/c6838924-901d-4bdb-9d6c-596571fe48ed/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALve_Lucas_Queiroz_etal_IOC_2024.pdfve_Lucas_Queiroz_etal_IOC_2024.pdfapplication/pdf1384032https://arca.fiocruz.br/bitstreams/ba7f2540-dc01-4d7e-b328-2d7e96a05a64/download8af1b6d302750874af341210894025d5MD52trueAnonymousREADTHUMBNAILcapa_Lucas_Queiroz_etal_IOC_2024.jpgcapa_Lucas_Queiroz_etal_IOC_2024.jpgCapaimage/jpeg2503336https://arca.fiocruz.br/bitstreams/9812253a-13f4-4065-b051-0dbccf0ebd90/download118459a04db1c103e616950868c111c0MD53falseAnonymousREADve_Lucas_Queiroz_etal_IOC_2024.pdf.jpgve_Lucas_Queiroz_etal_IOC_2024.pdf.jpgGenerated Thumbnailimage/jpeg6006https://arca.fiocruz.br/bitstreams/527abd7f-7d5a-4fdd-bf66-ecc8d606357d/downloadeaa9dadc14af28584b49225a1e70eca3MD57falseAnonymousREADTEXTve_Lucas_Queiroz_etal_IOC_2024.pdf.txtve_Lucas_Queiroz_etal_IOC_2024.pdf.txtExtracted texttext/plain71988https://arca.fiocruz.br/bitstreams/2cf51958-4f10-4316-abe7-1be9a9a51627/download7ebd69cf7b0e3ab5890971c4ceeb60c6MD56falseAnonymousREADicict/678392025-07-29 22:23:19.127open.accessoai:arca.fiocruz.br:icict/67839https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T01:23:19Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.none.fl_str_mv Novel isothermal nucleic acid amplification method for detecting malaria parasites
title Novel isothermal nucleic acid amplification method for detecting malaria parasites
spellingShingle Novel isothermal nucleic acid amplification method for detecting malaria parasites
Queiroz, Lucas Tavares de
Plasmodium detection
Isothermal nucleic acid amplifcation
Rapid diagnostic tests
Point-of-care
Brazilian Amazon
Malaria
03 Saúde e Bem-Estar
09 Indústria, inovação e infraestrutura
17 Parcerias e meios de implementação
title_short Novel isothermal nucleic acid amplification method for detecting malaria parasites
title_full Novel isothermal nucleic acid amplification method for detecting malaria parasites
title_fullStr Novel isothermal nucleic acid amplification method for detecting malaria parasites
title_full_unstemmed Novel isothermal nucleic acid amplification method for detecting malaria parasites
title_sort Novel isothermal nucleic acid amplification method for detecting malaria parasites
author Queiroz, Lucas Tavares de
author_facet Queiroz, Lucas Tavares de
Baptista, Barbara de Oliveira
Santos, Rebecca de Abreu Fernandes dos
Pereira, Carolina de Souza Faria
Lemos, Juliana Aline de Souza
Souza, Hugo Amorim dos Santos de
Martorano, Rodrigo Medeiros
Riccio, Evelyn Kety Pratt
Totino, Paulo Renato Rivas
Ferreira, Joseli de Oliveira
Lima Junior, Josué da Costa
Ribeiro, Cláudio Tadeu Daniel
Riccio, Lilian Rose Pratt
author_role author
author2 Baptista, Barbara de Oliveira
Santos, Rebecca de Abreu Fernandes dos
Pereira, Carolina de Souza Faria
Lemos, Juliana Aline de Souza
Souza, Hugo Amorim dos Santos de
Martorano, Rodrigo Medeiros
Riccio, Evelyn Kety Pratt
Totino, Paulo Renato Rivas
Ferreira, Joseli de Oliveira
Lima Junior, Josué da Costa
Ribeiro, Cláudio Tadeu Daniel
Riccio, Lilian Rose Pratt
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Queiroz, Lucas Tavares de
Baptista, Barbara de Oliveira
Santos, Rebecca de Abreu Fernandes dos
Pereira, Carolina de Souza Faria
Lemos, Juliana Aline de Souza
Souza, Hugo Amorim dos Santos de
Martorano, Rodrigo Medeiros
Riccio, Evelyn Kety Pratt
Totino, Paulo Renato Rivas
Ferreira, Joseli de Oliveira
Lima Junior, Josué da Costa
Ribeiro, Cláudio Tadeu Daniel
Riccio, Lilian Rose Pratt
dc.subject.en.none.fl_str_mv Plasmodium detection
Isothermal nucleic acid amplifcation
Rapid diagnostic tests
Point-of-care
Brazilian Amazon
Malaria
topic Plasmodium detection
Isothermal nucleic acid amplifcation
Rapid diagnostic tests
Point-of-care
Brazilian Amazon
Malaria
03 Saúde e Bem-Estar
09 Indústria, inovação e infraestrutura
17 Parcerias e meios de implementação
dc.subject.ods.none.fl_str_mv 03 Saúde e Bem-Estar
09 Indústria, inovação e infraestrutura
17 Parcerias e meios de implementação
description Produção científica do Laboratório de Imunoparasitologia.
publishDate 2024
dc.date.issued.fl_str_mv 2024
dc.date.accessioned.fl_str_mv 2025-01-02T14:44:48Z
dc.date.available.fl_str_mv 2025-01-02T14:44:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv QUEIROZ, Lucas Tavares de et al. Novel isothermal nucleic acid amplification method for detecting malaria parasites. Applied Microbiology and Biotechnology, v. 108, n. 544, p. 1-15, 27 Dec. 2024.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/67839
dc.identifier.issn.none.fl_str_mv 0175-7598
dc.identifier.doi.none.fl_str_mv 10.1007/s00253-024-13357-2
dc.identifier.eissn.none.fl_str_mv 1432-0614
identifier_str_mv QUEIROZ, Lucas Tavares de et al. Novel isothermal nucleic acid amplification method for detecting malaria parasites. Applied Microbiology and Biotechnology, v. 108, n. 544, p. 1-15, 27 Dec. 2024.
0175-7598
10.1007/s00253-024-13357-2
1432-0614
url https://arca.fiocruz.br/handle/icict/67839
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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