Local and systemic effects of BdipTX-I, a Lys-49 phospholipase A2 isolated from Bothrops diporus snake venom

Bibliographic Details
Main Author: Teixera, Leda Fabiélen
Publication Date: 2017
Other Authors: Carvalho, Letícia Helena de, Castro, Onássis Boeri de, Bastos, Jéssica Silva Félix, Néry, Neriane Monteiro, Oliveira, George Azevedo, Kayano, Anderson Makoto, Soares, Andreimar Martins, Zuliani, Juliana Pavan
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1016/j.toxicon.2017.11.007
Download full: https://arca.fiocruz.br/handle/icict/33448
Summary: O artigo encontra-se disponível para download no site do Editor.
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spelling Teixera, Leda FabiélenCarvalho, Letícia Helena deCastro, Onássis Boeri deBastos, Jéssica Silva FélixNéry, Neriane MonteiroOliveira, George AzevedoKayano, Anderson MakotoSoares, Andreimar MartinsZuliani, Juliana Pavan2019-06-10T19:58:07Z2019-06-10T19:58:07Z2017TEIXEIRA, Leda Fabiélen et al. Local and systemic effects of BdipTX-I, a Lys-49 phospholipase A2 isolated from Bothrops diporus snake venom. Toxicon, v. 141, p. 55-64, 2017.0041-0101https://arca.fiocruz.br/handle/icict/3344810.1016/j.toxicon.2017.11.0071879-3150O artigo encontra-se disponível para download no site do Editor.Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Centro de Biomoléculas Aplicadas à Saúde. Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Centro de Biomoléculas Aplicadas à Saúde. Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Centro de Biomoléculas Aplicadas à Saúde. Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil / Centro Universitário São Lucas. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Laboratório de Imunologia Celular Aplicada à Saúde. Porto Velho, RO, Brasil / Fundação Oswaldo Cruz. Centro de Biomoléculas Aplicadas à Saúde. Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.The present work aimed to isolate a basic phospholipase A2 (PLA2) from Bothrops diporus snake venom (BdV), evaluate and compare the myotoxic and oedema-inducing activities, as well as the systemic effects caused by both the isolated PLA2 and BdV on Swiss mice. A Lys-49 PLA2 (BdipTX-I) was obtained through two chromatographic steps: an ion-exchange and a reverse phase. The local (oedema and myotoxicity) and systemic (hepatic and renal functions) effects were then assessed for BdipTX-I and BdV. Results showed that the oedema-inducing activity was significant in all tested doses (5 and 20 mg/paw) for both BdipTX-I and BdV. Myotoxicity was evaluated by the increase of serum CK, CK-MB and LDH, and results showed that BdV effect is more prominent than BdipTX-I effect. The systemic effects were evaluated by determining specific laboratory markers: AST, ALT, GGT, ALP, urea, creatinine, protein and calcium. BdipTX-I and BdV were able to induce renal changes in the experimental model, leading to proteinuria (induced both by BdipTX-I and by BdV) and uremia (induced only by BdV). Thus, it is concluded that the systemic effects of BdV and BdipTX-I occur differently.engElsevierLocal and systemic effects of BdipTX-I, a Lys-49 phospholipase A2 isolated from Bothrops diporus snake venominfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePhospholipase A2Bothrops diporusOedemaMyotoxicitySystemic effectinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/de231078-3270-41f5-a185-a3cdb2fef277/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALLocal and systemic effects of BdipTX-I, a Lys-49 phospholipase A 2 isolated from Bothrops diporus snake venom.pdfLocal and systemic effects of BdipTX-I, a Lys-49 phospholipase A 2 isolated from Bothrops diporus snake venom.pdfapplication/pdf1282792https://arca.fiocruz.br/bitstreams/18ab2eb9-4d7b-4397-9c94-109780b86cd2/download8aa07499a401deadab8915b31c39f78dMD52trueAnonymousREADTEXTLocal and systemic effects of BdipTX-I, a Lys-49 phospholipase A 2 isolated from Bothrops diporus snake venom.pdf.txtLocal and systemic effects of BdipTX-I, a Lys-49 phospholipase A 2 isolated from Bothrops diporus snake venom.pdf.txtExtracted texttext/plain58587https://arca.fiocruz.br/bitstreams/c3fab8d1-ea2c-43aa-bed9-3bcfb73fdd32/downloadd808c025ee4c4d0c4456368761eb9f5eMD55falseAnonymousREADTHUMBNAILLocal and systemic effects of BdipTX-I, a Lys-49 phospholipase A 2 isolated from Bothrops diporus snake venom.pdf.jpgLocal and systemic effects of BdipTX-I, a Lys-49 phospholipase A 2 isolated from Bothrops diporus snake venom.pdf.jpgGenerated Thumbnailimage/jpeg6143https://arca.fiocruz.br/bitstreams/860b8981-4b65-446c-8afc-2de12cb7f6f3/downloadf9dd11cbf90b1cd1dede3b8cbf687d53MD56falseAnonymousREADicict/334482025-07-30 02:53:06.462open.accessoai:arca.fiocruz.br:icict/33448https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T05:53:06Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.none.fl_str_mv Local and systemic effects of BdipTX-I, a Lys-49 phospholipase A2 isolated from Bothrops diporus snake venom
title Local and systemic effects of BdipTX-I, a Lys-49 phospholipase A2 isolated from Bothrops diporus snake venom
spellingShingle Local and systemic effects of BdipTX-I, a Lys-49 phospholipase A2 isolated from Bothrops diporus snake venom
Teixera, Leda Fabiélen
Phospholipase A2
Bothrops diporus
Oedema
Myotoxicity
Systemic effect
title_short Local and systemic effects of BdipTX-I, a Lys-49 phospholipase A2 isolated from Bothrops diporus snake venom
title_full Local and systemic effects of BdipTX-I, a Lys-49 phospholipase A2 isolated from Bothrops diporus snake venom
title_fullStr Local and systemic effects of BdipTX-I, a Lys-49 phospholipase A2 isolated from Bothrops diporus snake venom
title_full_unstemmed Local and systemic effects of BdipTX-I, a Lys-49 phospholipase A2 isolated from Bothrops diporus snake venom
title_sort Local and systemic effects of BdipTX-I, a Lys-49 phospholipase A2 isolated from Bothrops diporus snake venom
author Teixera, Leda Fabiélen
author_facet Teixera, Leda Fabiélen
Carvalho, Letícia Helena de
Castro, Onássis Boeri de
Bastos, Jéssica Silva Félix
Néry, Neriane Monteiro
Oliveira, George Azevedo
Kayano, Anderson Makoto
Soares, Andreimar Martins
Zuliani, Juliana Pavan
author_role author
author2 Carvalho, Letícia Helena de
Castro, Onássis Boeri de
Bastos, Jéssica Silva Félix
Néry, Neriane Monteiro
Oliveira, George Azevedo
Kayano, Anderson Makoto
Soares, Andreimar Martins
Zuliani, Juliana Pavan
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Teixera, Leda Fabiélen
Carvalho, Letícia Helena de
Castro, Onássis Boeri de
Bastos, Jéssica Silva Félix
Néry, Neriane Monteiro
Oliveira, George Azevedo
Kayano, Anderson Makoto
Soares, Andreimar Martins
Zuliani, Juliana Pavan
dc.subject.en.none.fl_str_mv Phospholipase A2
Bothrops diporus
Oedema
Myotoxicity
Systemic effect
topic Phospholipase A2
Bothrops diporus
Oedema
Myotoxicity
Systemic effect
description O artigo encontra-se disponível para download no site do Editor.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2019-06-10T19:58:07Z
dc.date.available.fl_str_mv 2019-06-10T19:58:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TEIXEIRA, Leda Fabiélen et al. Local and systemic effects of BdipTX-I, a Lys-49 phospholipase A2 isolated from Bothrops diporus snake venom. Toxicon, v. 141, p. 55-64, 2017.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/33448
dc.identifier.issn.none.fl_str_mv 0041-0101
dc.identifier.doi.none.fl_str_mv 10.1016/j.toxicon.2017.11.007
dc.identifier.eissn.none.fl_str_mv 1879-3150
identifier_str_mv TEIXEIRA, Leda Fabiélen et al. Local and systemic effects of BdipTX-I, a Lys-49 phospholipase A2 isolated from Bothrops diporus snake venom. Toxicon, v. 141, p. 55-64, 2017.
0041-0101
10.1016/j.toxicon.2017.11.007
1879-3150
url https://arca.fiocruz.br/handle/icict/33448
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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