Studying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi

Bibliographic Details
Main Author: Vieira, Cecilia Stahl
Publication Date: 2011
Other Authors: Almeida, Diogo Burigo, Thomaz, André Alexandre de, Menna-Barreto, Rubem Figueredo Sadok, Santos-Mallet, Jacenir R., Cesar, Carlos Lenz, Gomes, Suzete Araujo Oliveira, Feder, Denise
Format: Article
Language: por
Source: Repositório Institucional da FIOCRUZ (ARCA)
Download full: https://arca.fiocruz.br/handle/icict/10674
Summary: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmanioses. Setor de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.
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spelling Vieira, Cecilia StahlAlmeida, Diogo BurigoThomaz, André Alexandre deMenna-Barreto, Rubem Figueredo SadokSantos-Mallet, Jacenir R.Cesar, Carlos LenzGomes, Suzete Araujo OliveiraFeder, Denise2015-06-08T14:01:45Z2015-06-08T14:01:45Z2011VIEIRA, Cecilia Stahl et al. Studying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi. Mem Inst Oswaldo Cruz, v.106, n.2, p.158-165, Mar. 2011.1678-8060https://arca.fiocruz.br/handle/icict/10674porFundação Oswaldo CruzStudying nanotoxic effects of CdTe quantum dots in Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmanioses. Setor de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.Universidade Estadual de Campinas.Instituto de Física Gleb Wataghin. Laboratório de Aplicações Biomédicas de Lasers. Campinas, SP, Brasil.Universidade Estadual de Campinas.Instituto de Física Gleb Wataghin. Laboratório de Aplicações Biomédicas de Lasers. Campinas, SP, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmanioses. Setor de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.Universidade Estadual de Campinas.Instituto de Física Gleb Wataghin. Laboratório de Aplicações Biomédicas de Lasers. Campinas, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmanioses. Setor de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, BrasilUniversidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, BrasilSemiconductor nanoparticles, such as quantum dots (QDs), were used to carry out experiments in vivo and ex vivo with Trypanosoma cruzi. However, questions have been raised regarding the nanotoxicity of QDs in living cells, microorganisms, tissues and whole animals. The objective of this paper was to conduct a QD nanotoxicity study on living T. cruzi protozoa using analytical methods. This was accomplished using in vitro experiments to test the interference of the QDs on parasite development, morphology and viability. Our results show that after 72 h, a 200 μM cadmium telluride (CdTe) QD solution induced important morphological alterations in T. cruzi, such as DNA damage, plasma membrane blebbing and mitochondrial swelling. Flow cytometry assays showed no damage to the plasma membrane when incubated with 200 μM CdTe QDs for up to 72 h (propidium iodide cells), giving no evidence of classical necrosis. Parasites incubated with 2 μM CdTe QDs still proliferated after seven days. In summary, a low concentration of CdTe QDs (2 μM) is optimal for bioimaging, whereas a high concentration (200 μM CdTe) could be toxic to cells. Taken together, our data indicate that 2 μM QD can be used for the successful long-term study of the parasite-vector interaction in real time.CdTe quantum dotsTrypanosoma cruziNanotoxicityTrypanosoma cruziinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://arca.fiocruz.br/bitstreams/d3e3bfef-46a7-4493-bbe1-4a45e9210f5c/download7d48279ffeed55da8dfe2f8e81f3b81fMD51falseAnonymousREADORIGINALcecilia_vieiraetal_IOC_2011.pdfapplication/pdf793107https://arca.fiocruz.br/bitstreams/f14f460e-ed42-4d10-ab97-b2fb208de094/download2e595bd49a2ee63b35ea078b6679066bMD52trueAnonymousREADTEXTcecilia_vieiraetal_IOC_2011.pdf.txtcecilia_vieiraetal_IOC_2011.pdf.txtExtracted texttext/plain35894https://arca.fiocruz.br/bitstreams/2023a280-8027-44ad-a81d-3d120ea6aa05/download51980de540efd97698f194ce64b125afMD57falseAnonymousREADTHUMBNAILcecilia_vieiraetal_IOC_2011.pdf.jpgcecilia_vieiraetal_IOC_2011.pdf.jpgGenerated Thumbnailimage/jpeg5939https://arca.fiocruz.br/bitstreams/d3904331-6a19-45f7-8756-1e3f93f8fe90/downloadc8691ee02252dc2fbcb92ee22a51fd3cMD58falseAnonymousREADicict/106742025-07-29 18:24:21.555open.accessoai:arca.fiocruz.br:icict/10674https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-29T21:24:21Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)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
dc.title.none.fl_str_mv Studying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi
title Studying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi
spellingShingle Studying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi
Vieira, Cecilia Stahl
CdTe quantum dots
Trypanosoma cruzi
Nanotoxicity
Trypanosoma cruzi
title_short Studying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi
title_full Studying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi
title_fullStr Studying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi
title_full_unstemmed Studying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi
title_sort Studying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi
author Vieira, Cecilia Stahl
author_facet Vieira, Cecilia Stahl
Almeida, Diogo Burigo
Thomaz, André Alexandre de
Menna-Barreto, Rubem Figueredo Sadok
Santos-Mallet, Jacenir R.
Cesar, Carlos Lenz
Gomes, Suzete Araujo Oliveira
Feder, Denise
author_role author
author2 Almeida, Diogo Burigo
Thomaz, André Alexandre de
Menna-Barreto, Rubem Figueredo Sadok
Santos-Mallet, Jacenir R.
Cesar, Carlos Lenz
Gomes, Suzete Araujo Oliveira
Feder, Denise
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vieira, Cecilia Stahl
Almeida, Diogo Burigo
Thomaz, André Alexandre de
Menna-Barreto, Rubem Figueredo Sadok
Santos-Mallet, Jacenir R.
Cesar, Carlos Lenz
Gomes, Suzete Araujo Oliveira
Feder, Denise
dc.subject.en.none.fl_str_mv CdTe quantum dots
Trypanosoma cruzi
Nanotoxicity
topic CdTe quantum dots
Trypanosoma cruzi
Nanotoxicity
Trypanosoma cruzi
dc.subject.decs.none.fl_str_mv Trypanosoma cruzi
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmanioses. Setor de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2015-06-08T14:01:45Z
dc.date.available.fl_str_mv 2015-06-08T14:01:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VIEIRA, Cecilia Stahl et al. Studying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi. Mem Inst Oswaldo Cruz, v.106, n.2, p.158-165, Mar. 2011.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/10674
dc.identifier.issn.none.fl_str_mv 1678-8060
identifier_str_mv VIEIRA, Cecilia Stahl et al. Studying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi. Mem Inst Oswaldo Cruz, v.106, n.2, p.158-165, Mar. 2011.
1678-8060
url https://arca.fiocruz.br/handle/icict/10674
dc.language.iso.fl_str_mv por
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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