Biblioteca Digital de Teses e Dissertações da USP 1

Tavares, Lucas Alves 1

Dissertação 1

Português 1

?2 depletion 1 AP-1 1 AP-1 subunits. By pull-down technique 1 AP-2 1 AP-2 and AP-3 are potentially heterogeneous due to the existence of multiple subunits isoforms encoded by different genes. However 1 AP-3 complexes and Nef does not appear to interact with AP-4 and AP-5 subunits. However 1 Mais ... ESCRT 1 HIV promotes several changes in the physiology of the host cell to promote their survival and enhance replication. The fast progression of HIV-1 in hu 1 HIV-1 1 MVB 1 Nef 1 The Human Immunodeficiency Virus (HIV) is the etiologic agent of Acquired Immunodeficiency Syndrome (AIDS). AIDS is a disease which has a global distr 1 acts in the CD4 down-regulation induced by Nef. These studies contribute to a better understanding on the molecular mechanisms involved in Nef activit 1 although not need charging ubiquitination 1 and it is estimated that there are currently at least 36.9 million people infected with the virus. During the replication cycle 1 and only the AP-1 variant comprising ?2 1 another subunit of AP-1 1 being an important protein in the endo-lysosomal system. Furthermore 1 but also seem to compose AP-1 complex with distinct cell functions 1 but not ?1 1 but not ?1 depletion 1 but the molecular mechanisms are still incompletely understood. Nef forms a tripartite complex with the cytosolic tail of the CD4 and adapter protein 1 by flow cytometry assay 1 compromises the reduction of surface CD4 levels induced by Nef. Immunofluorescence microscopy analysis also revealed that ?2 depletion impairs the red 1 compromising the efficient CD4 degradation by Nef. Moreover 1 depends on the proteins from ESCRTs (Endosomal Sorting Complexes Required for Transport) machinery and the action of Alix, an accessory protein ESCRT 1 inducing CD4 internalization and lysosome degradation. Previous research has demonstrated that CD4 target to lysosomes by Nef involves targeting of th 1 internalized CD4 accumulates in enlarged HRS-GFP positive endosomes 1 is required in Nef-mediated targeting of CD4 to lysosomes and the ?2 participation in this process is conserved by Nef from different viral strains. F 1 not ?1-adaptin 1 Ver todos ... menos ...