MiR-20a is upregulated in serum from domestic feline with PKD1 mutation
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| 1. autor: | |
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| Data wydania: | 2022 |
| Kolejni autorzy: | , , , , |
| Format: | Article |
| Język: | eng por |
| Źródło: | Repositório Institucional da UnB |
| Download full: | https://doi.org/10.1371/journal.pone.0279337 http://repositorio.unb.br/handle/10482/54774 https://orcid.org/0000-0002-9112-1718 |
Streszczenie: | Polycystic kidney disease (PKD), also known as autosomal dominant polycystic kidney disease (ADPKD) is a genetically heterogeneous condition characterized by cysts in renal parenchyma. It is the most prevalent inherited disease of domestic cats. MicroRNAs (miRNAs or ncRNA) are short, noncoding, single-stranded RNAs that may induce PKD cytogenesis by affecting numerous targets genes as well as by directly regulating PKD gene expression. We compared the relative expression profile of miR-20a, -192, -365, -15b-5p, and -16-5p from plasma and serum samples of nine domestic cats with PKD1 mutation, detected by polymerase chain reaction (PCR), and a control group (n = 10). Blood samples from cats with PKD1 mutation provide similar concentrations of microRNAs either from plasma or serum. Serum miR-20a is upregulated in PKD group with p < 0.005; Roc curve analysis showed an AUC of 90,1% with a cut-off value sensitivity of 77.8% and specificity of 100%. This data provides important information regarding renal miRNA expression in peripheral blood sampling. |