Ramularia leaf spot: PCR‑based methods reveal widespread distribution of Ramulariopsis pseudoglycines and limited presence of R. gossypii in Brazil
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| Hlavní autor: | |
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| Datum vydání: | 2023 |
| Další autoři: | , , , , , |
| Médium: | Article |
| Jazyk: | eng |
| Zdroj: | Repositório Institucional da UnB |
| Download full: | http://repositorio.unb.br/handle/10482/46863 https://doi.org/10.1038/s41598-023-33530-3 https://orcid.org/0000-0002-0550-5847 https://orcid.org/0000-0001-5195-9930 https://orcid.org/0000-0002-8166-7127 https://orcid.org/0000-0002-5204-2961 https://orcid.org/0000-0002-5798-4552 https://orcid.org/0000-0003-0257-1539 https://orcid.org/0000-0003-2624-302X |
Shrnutí: | Whilst Brazil is the fourth largest cotton producer globally, incidence of ramularia leaf spot (RLS) has decreased yield. In 2017–18 and 2018–19, ca. 300 fungal samples were collected throughout Brazil. Hyphal tip cultures were obtained for amplification of the RNA polymerase II (RPB2), 28S rRNA, the ribosomal DNA internal transcribed spacers (ITS), actin (ACT), elongation factor (EF1-α) and histone H3 (HIS3) genomic regions. Additionally, sequences of the glyceraldehyde‑3‑phosphate dehydrogenase (GAPDH) were obtained by nanopore sequencing and the EF1-α region was selected as a marker for rapid recognition of Ramulariopsis species. Clade assignments based on the concatenated‑sequence tree were identical to those in tree generated by RPB2‑sequences, as well as in an RPB2 haplotype network and an ISSR (TGTC) 4 dendrogram, in identification with species‑specific primers and based on morphological comparisons. Out of 267 examined isolates, 252 were identified as Ramulariopsis pseudoglycines, indicating this species as the most widespread causal agent of cotton RLS in the Brazilian growing regions. Species‑specific primers developed in the study that target the EF1-α gene provide an opportunity for extensive RLS sampling worldwide to study the distribution of Ramulariopsis species. Such data will aid breeders and plant pathologists in cotton disease resistance development and fungicide resistance avoidance. |
Internet
http://repositorio.unb.br/handle/10482/46863https://doi.org/10.1038/s41598-023-33530-3
https://orcid.org/0000-0002-0550-5847
https://orcid.org/0000-0001-5195-9930
https://orcid.org/0000-0002-8166-7127
https://orcid.org/0000-0002-5204-2961
https://orcid.org/0000-0002-5798-4552
https://orcid.org/0000-0003-0257-1539
https://orcid.org/0000-0003-2624-302X